LOCUS       sequence-161713-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of SSA1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP84
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    648..681
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga
       61 ggatcccata ttatctgtta tttacttgaa tttttgtttc ttgtaatact tgattacttt
      121 tcttttgatg tgcttatctt acaaatagag aaaataaaac aacttaagta agaattggga
      181 aacgaaacta caactcaatc ccttctcgaa gatacatcaa tccacccctt atataacctt
      241 gaagtcctcg aaacgatcag ctaatctaaa tggcccccct tctttttggg ttctttctct
      301 cccttttgcc gccgatggaa cgttctggaa aaagaagaat aatttaatta ctttctcaac
      361 taaaatctgg agaaaaaacg caaatgacag cttctaaacg ttccgtgtgc tttctttcta
      421 gaatgttctg gaaagtttac aacaatccac aagaacgaaa atgccgttga caatgatgaa
      481 accatcatcc acacaccgcg cacacgtgct ttatttcttt ttctgaattt tttttttccg
      541 ccattttcaa ccaaggaaat tttttttctt agggctcaga acctgcaggt gaagaagcgc
      601 tttagaaatc aaagcacaac gtaacaattt gtcgagcaac ccttaatata acttcgtata
      661 atgtatgcta tacgaagtta ttaggtctag agatcccaat acaacagatc acgtgatctt
      721 tttgtaagat gaagttgaag tagtgttgca ccgtgccaat gcaggtggct attagattaa
      781 atatgtgatt tgttctatta agtttcctgt ataattaatg gggagcgctg attctctttt
      841 ggtacgcttc ccatccagca tttctgtatc tttcaccttc aaccttagga tctctaccct
      901 tggcgaaaag tcctctgcca acaatgatga tatctgatcc accacttaca acttcgtcga
      961 cggttctgta ctgctgaccc aatgcatcg
//
