LOCUS       sequence-161715-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with 
            minimal promoter plus luciferase reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: HOP-flash
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(437..455)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
       61 cagaacattt ctctatcgat aggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc gcccttggtg gggaggaatg cgaggaatgt ccctgtttgt ggtggggagg
      181 aatgcgagga atgtccctgt ttgtggtggg gaggaatgcg aggaatgtcc ctgtttgtgg
      241 tggggaggaa tgcgaggaat gtccctgttt aagggcgaat tctgcagatt agagggtata
      301 taatggatcc ggtactcgag atctgcgatc taagtaagct tggcattccg gtactgttgg
      361 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      421 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      481 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      541 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      601 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      661 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      721 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
      781 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
      841 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
      901 tgaatacgat tttgtgccag agtccttcga tagggacaag
//
