LOCUS       sequence-161718-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL4A promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP86
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    730..763
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccatttcgat gattgttctt gggatattgc taaattacaa gaaaagtgat
      121 gaaaagtata agttgcgagg ctaaaagaaa tgtgatggag ataccattcg tttatataaa
      181 tttttttttt tttttttttt cagattgaaa aaaaattttt cacttctcac tgtgcgtcgt
      241 gtgacgtgat attaatatag taaaagtgca tattcaatat tatttaggat agtctcttga
      301 gacatctatc taagatatta tgctccgatt gtgcaagtgt tcgagcattt tggagcatgc
      361 ttttagtagc acttgttatc tttgacgagt tgtcgccttt tatgcaaggt agttgctttt
      421 ccttaagtaa tttggaaagc aatttcttgg aatggggatt gtcttttttg gccaggaagg
      481 gcagcttttc ttcgttaact atagaatcac tgatgtgagg agtggccttt ttggagaaca
      541 atgattccca ttgaacggcc gttaaagtca aaaggttagc taaggcccac gttgagggtg
      601 aaggctcgcc acttgagtct atgtaaagct gggaaagcca tgattccaag ctgtcatcac
      661 cctcttcctc ttcctcttcg ctctctatat cgtcagacag attgtctgat ttgtcgagca
      721 acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct agagatccca
      781 atacaacaga tcacgtgatc ttttgtaaga tgaagttgaa gtagtgttgc accgtgccaa
      841 tgcaggtggc tattagatta aatatgtgat ttgttctatt aagtttcctg tataattaat
      901 ggggagcgct gattctcttt tggtacgctt cccatccagc atttctgtat ctttcacctt
      961 caaccttagg at
//
