LOCUS       sequence-161721-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL4A promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP86
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(223..933)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 gcatttaaat cattacgacc gagattcccg ggtaataact gatataatta aattgaagct
       61 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
      121 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
      181 gcatcccttc cctttgcaaa tagtcctact agtttaagat cttttgtaca attcatccat
      241 accatgggta ataccagcag cagtaacaaa ttctaacaag accatgtggt ctctcttttc
      301 gtttggatct ttggataagg cagattgagt ggataagtaa tggttgtctg gtaacaagac
      361 tggaccatca ccaattggag tattttgttg ataatggtca gctaattgaa cagaaccatc
      421 ttcaatgttg tgtctaattt tgaagttaac tttgatacca ttcttttgtt tgtcagccat
      481 gatgtaaaca ttgtgagagt tatagttgta ttccaatttg tgacctaaaa tgttaccatc
      541 ttctttaaaa tcaatacctt ttaattcgat tctattaact aaggtatcac cttcaaactt
      601 gacttcagct ctggtcttgt agttaccgtc atctttgaaa aaaatagttc tttcttgaac
      661 ataaccttct ggcatggcag acttgaaaaa gtcatgttgt ttcatatgat ctgggtatct
      721 cgcaaaacat tgaacaccat aaccgaaagt agtgactaag gttggccatg gaactggcaa
      781 tttaccagta gtacaaataa attttaaggt caatttaccg taagtagcat caccttcacc
      841 ttcaccggag acagaaaatt tgtgaccatt aacatcacca tctaattcaa ccaaaattgg
      901 gacaacacca gtgaataatt cttcaccttt agaggatccc atttcgatga ttgttcttgg
      961 gatattgcta aattacaaga aaagtgatga aaagtataag tt
//
