LOCUS       sequence-161730-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TEF1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPE9
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(226..936)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 catgcattta aatcattacg accgagattc ccgggtaata actgatataa ttaaattgaa
       61 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
      121 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
      181 ttagcatccc ttccctttgc aaatagtcct actagtttaa gatcttttgt acaattcatc
      241 cataccatgg gtaataccag cagcagtaac aaattctaac aagaccatgt ggtctctctt
      301 ttcgtttgga tctttggata aggcagattg agtggataag taatggttgt ctggtaacaa
      361 gactggacca tcaccaattg gagtattttg ttgataatgg tcagctaatt gaacagaacc
      421 atcttcaatg ttgtgtctaa ttttgaagtt aactttgata ccattctttt gtttgtcagc
      481 catgatgtaa acattgtgag agttatagtt gtattccaat ttgtgaccta aaatgttacc
      541 atcttcttta aaatcaatac cttttaattc gattctatta actaaggtat caccttcaaa
      601 cttgacttca gctctggtct tgtagttacc gtcatctttg aaaaaaatag ttctttcttg
      661 aacataacct tctggcatgg cagacttgaa aaagtcatgt tgtttcatat gatctgggta
      721 tctcgcaaaa cattgaacac cataaccgaa agtagtgact aaggttggcc atggaactgg
      781 caatttacca gtagtacaaa taaattttaa ggtcaattta ccgtaagtag catcaccttc
      841 accttcaccg gagacagaaa atttgtgacc attaacatca ccatctaatt caaccaaaat
      901 tgggacaaca ccagtgaata attcttcacc tttagaggat cccattttct cgagtttgta
      961 attaaaactt agattagatt gctatgcttt ctttctaatg agcaagaagt aaaaaaagtt
     1021 gtaatagaac aagaaaaatg aaactg
//
