LOCUS       sequence-161742-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TEF2 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP90
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga
       61 ggatcccatg tttagttaat tatagttcgt tgaccatata ttctaaaaac aagtactcct
      121 taaaaaaaaa ccttgaaggg aataaacaag tagaatagat agagagaaaa atagaaaatg
      181 caagagaatt tatatattag aaagagagaa agaaaaatgg aaaaaaaaaa ataggaaaag
      241 ccagaaatag cactagaagg agcgacacca gaaaagaagg tgatggaacc aatttagcta
      301 tatatagtta actaccggct cgatcatctc tgcctccagc atagtcgaag aagaattttt
      361 tttttcttga ggcttctgtc agcaactcgt attttttctt tcttttttgg tgagcctaaa
      421 aagttcccac gttctcttgt acgacgccgt cacaaacaac cttatgggta atttgtcgcg
      481 gtctgggtgt ataaatgtgt gggtgcaaca tgaatgtacg gaggtagttt gctgattggc
      541 ggtctataga taccttggtt atggcgccct cacagccggc aggggaagcg cctacgcttg
      601 acatctacta tatgtaagta tacggcccca tatatatata tatatatata cattaaacat
      661 tattggtaaa tacaccagca accgcatgat tgatgttatg gtggaatata ggtagctaaa
      721 aaaactctac ataacaaagt gtcgagcaac ccttaatata acttcgtata atgtatgcta
      781 tacgaagtta ttaggtctag agatcccaat acaacagatc acgtgatctt tttgtaagat
      841 gaagttgaag tagtgttgca ccgtgccaat gcaggtggct attagattaa atatgtgatt
      901 tgttctatta agtttcctgt ataattaatg gggagcgctg attctctttt ggtacgcttc
      961 ccatccagca tttctgtatc tttcaccttc aaccttagga tctctaccct tgg
//
