LOCUS       sequence-161744-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TEF2 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP90
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(224..934)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tgcatttaaa tcattacgac cgagattccc gggtaataac tgatataatt aaattgaagc
       61 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
      121 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
      181 agcatccctt ccctttgcaa atagtcctac tagtttaaga tcttttgtac aattcatcca
      241 taccatgggt aataccagca gcagtaacaa attctaacaa gaccatgtgg tctctctttt
      301 cgtttggatc tttggataag gcagattgag tggataagta atggttgtct ggtaacaaga
      361 ctggaccatc accaattgga gtattttgtt gataatggtc agctaattga acagaaccat
      421 cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc attcttttgt ttgtcagcca
      481 tgatgtaaac attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccat
      541 cttctttaaa atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact
      601 tgacttcagc tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa
      661 cataaccttc tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc
      721 tcgcaaaaca ttgaacacca taaccgaaag tagtgactaa ggttggccat ggaactggca
      781 atttaccagt agtacaaata aattttaagg tcaatttacc gtaagtagca tcaccttcac
      841 cttcaccgga gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg
      901 ggacaacacc agtgaataat tcttcacctt tagaggatcc catgtttagt taattatagt
      961 tcgttgacca tatattctaa aaacaagtac tccttaaaaa aaaaccttga
//
