LOCUS       sequence-161746-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Campylobacter jejuni Cas9 (Cje Cas9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Ma E, Harrington LB, O'Connell MR, Zhou K, Doudna JA
  TITLE     Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):398-407. doi: 
            10.1016/j.molcel.2015.10.030.
  PUBMED    26545076
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "398-407"
COMMENT     Sequence Label: pEC-K-CBP_CjeCas9
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..84
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggactaaaga gaagatggaa aaagaatttc atagccgtct cagcagccaa ccgctttaag
       61 aaaatctcat cctccggggc acttactagt gaaaacctgt atttccaggg agcagcctcg
      121 atggcaagaa ttttggcatt tgatatagga ataagctcta ttggctgggc atttagtgaa
      181 aatgatgagt taaaagattg tggagttagg atttttacaa aggttgaaaa tcccaaaaca
      241 ggagaatcct tagccttgcc aagacgcctt gcaagaagtg ctagaaaacg ccttgctaga
      301 cgcaaagcaa ggctaaatca cttaaaacat ttaatcgcaa atgaatttaa gttaaattac
      361 gaagattatc aaagctttga tgaaagttta gccaaggctt ataaaggatc tttaattagc
      421 ccttatgagt taagatttag agctttaaac gagcttttaa gtaagcaaga ttttgcaaga
      481 gtgattttac atattgcaaa aaggcgtggc tatgatgata ttaaaaatag tgatgataaa
      541 gaaaaaggtg cgattttaaa agctatcaag caaaatgaag aaaaactagc aaattatcaa
      601 agtgtgggcg agtatcttta taaagagtat tttcaaa
//
