LOCUS       sequence-161749-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of PGK1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP91
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    707..740
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
       61 tagaggatcc cattgtttta tatttgttgt aaaaagtaga taattacttc cttgatgatc
      121 tgtaaaaaag agaaaaagaa agcatctaag aacttgaaaa actacgaatt agaaaagacc
      181 aaatatgtat ttcttgcatt gaccaattta tgcaagttta tatatatgta aatgtaagtt
      241 tcacgaggtt ctactaaact aaaccacccc cttggttaga agaaaagagt gtgtgagaac
      301 aggctgttgt tgtcacacga ttcggacaat tctgtttgaa agagagagag taacagtacg
      361 atcgaacgaa ctttgctctg gagatcacag tgggcatcat agcatgtggt actaaaccct
      421 ttcccgccat tccagaacct tcgattgctt gttacaaaac ctgtgagccg tcgctaggac
      481 cttgttgtgt gacgaaattg gaagctgcaa tcaataggaa gacaggaagt cgagcgtgtc
      541 tgggtttttt cagttttgtt ctttttgcaa acaaatcacg agcgacggta atttctttct
      601 cgataagagg ccacgtgctt tatgagggta acatcaattc aagaaggagg gaaacacttc
      661 ctttttctgg ccctgataat agtatgaggg tcgagcaacc cttaatataa cttcgtataa
      721 tgtatgctat acgaagttat taggtctaga gatcccaata caacagatca cgtgatcttt
      781 ttgtaagatg aagttgaagt agtgttgcac cgtgccaatg caggtggcta ttagattaaa
      841 tatgtgattt gttctattaa gtttcctgta taattaatgg ggagcgctga ttctcttttg
      901 gtacgcttcc catccagcat ttctgtatct ttcaccttca accttaggat ctctaccctt
      961 ggcgaaaagt cctctgccaa caatgatgat atctgatcca ccacttacaa cttcgtcgac
     1021 ggttctgtac tgctgaccca atgcatcgcc tttgtcgt
//
