LOCUS       sequence-161756-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM
  TITLE     Snail1, Snail2, and E47 promote mammary epithelial branching 
            morphogenesis.
  JOURNAL   EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159.
  PUBMED    21610693
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-24- 24";
            volume: "30"; issue: "13"; pages: "2662-74"
COMMENT     Sequence Label: p3xFLAG-EYFP
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(130..132,133..135,136..648)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(175..196)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(436..455)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      121 gcgaattcaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      181 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      241 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      301 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
      361 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      421 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      481 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      541 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      601 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      661 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      721 gacaaccact a
//
