LOCUS       sequence-161761-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TDH3 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP92
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(75..741)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(182..205)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     protein_bind    825..858
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccattttgtt tgtttatgtg tgtttattcg aaactaagtt cttggtgttt
      121 taaaactaaa aaaaagacta actataaaag tagaatttaa gaagtttaag aaatagattt
      181 acagaattac aatcaatacc taccgtcttt atatacttat tagtcaagta ggggaataat
      241 ttcagggaac tggtttcaac cttttttttc agctttttcc aaatcagaga gagcagaagg
      301 taatagaagg tgtaagaaaa tgagatagat acatgcgtgg gtcaattgcc ttgtgtcatc
      361 atttactcca ggcaggttgc atcactccat tgaggttgtg cccgtttttt gcctgtttgt
      421 gcccctgttc tctgtagttg cgctaagaga atggacctat gaactgatgg ttggtgaaga
      481 aaacaatatt ttggtgctgg gattcttttt ttttctggat gccagcttaa aaagcgggct
      541 ccattatatt tagtggatgc caggaataaa ctgttcaccc agacacctac gatgttatat
      601 attctgtgta acccgccccc tattttgggc atgtacgggt tacagcagaa ttaaaaggct
      661 aattttttga ctaaataaag ttaggaaaat cactactatt aattatttac gtattctttg
      721 aaatggcagt attgataatg ataaactcga actgaaaaag cgtgtttttt attcaaaatg
      781 attctaactc ccttacgtaa tcaaggagtc gagcaaccct taatataact tcgtataatg
      841 tatgctatac gaagttatta ggtctagaga tcccaataca acagatcacg tgatcttttt
      901 gtaagatgaa gttgaagtag tgttgcaccg tgccaatgca ggtggctatt agattaaata
      961 tgtgatttgt tctattaagt ttcctgtata attaatgggg agcgctgatt ctcttttggt
     1021 acgcttccca tccagcatt
//
