LOCUS       sequence-161768-       12934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMygro
FEATURES             Location/Qualifiers
     source          1..12934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5641..5643,5644..5646,5647..6360)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5641..5643,5644..5646,5647..6357)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5686..5707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5947..5966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6294..6315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6368..6942,6943..6945,6946..6954)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6535..6552)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6762..6781
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6964..7989
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12290..12309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12291..12309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12319..12336)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12319..12335)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12328..12350)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12476..12931)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12613..12634)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12825..12844
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgcgt
     5641 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5701 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5761 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5821 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5881 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5941 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6001 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6061 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6121 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6181 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6241 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6301 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6361 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6421 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6481 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6541 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6601 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6661 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6721 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6781 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6841 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6901 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggcg
     6961 cgtatgaaaa agcccgagct gactgccacc tctgtcgaga agttcctgat tgaaaagttt
     7021 gattccgtga gtgacctgat gcagctgagc gagggcgagg aatcccgggc cttctctttt
     7081 gacgtgggcg gaaggggcta cgtgctgaga gtcaactctt gcgccgacgg attctataag
     7141 gatcgctacg tgtatcgaca ctttgctagt gccgctctgc ccatccctga ggtgctggac
     7201 attggcgagt tcagcgaatc cctgacatac tgtatctcca ggagagccca gggagtgact
     7261 ctgcaggatc tgcctgagac cgaactgcct gccgtgctgc agcctgtggc tgaagctatg
     7321 gacgctatcg cagccgctga tctgtcacag actagcgggt tcggcccatt tggcccccag
     7381 ggaattgggc agtacaccac atggagagac tttatctgcg caattgccga tccccacgtg
     7441 tatcattggc agacagtcat ggacgatacc gtgagcgcca gtgtcgctca ggcactggac
     7501 gagctgatgc tgtgggcaga ggattgtcct gaagtgaggc acctggtcca tgctgacttc
     7561 gggagcaaca atgtgctgac cgataacggc agaatcacag ccgtcattga ctggagtgag
     7621 gctatgtttg gcgattcaca gtacgaagtg gctaatatct tcttttggcg accctggctg
     7681 gcatgcatgg agcagcagac ccgctatttc gagcggcgcc atcctgaact ggcagggtct
     7741 ccaagactgc gggcctacat gctgcggatt ggcctggacc agctgtatca gagcctggtg
     7801 gatggaaatt ttgacgatgc agcatgggca caggggcggt gcgacgctat cgtgcgcagc
     7861 ggagcaggaa ctgtgggaag aacccagatt gcccgaaggt ccgctgcagt gtggacagat
     7921 gggtgcgtgg aggtgctggc agatagcgga aataggagac caagtacaag accacgggca
     7981 aaagagtaat ctagacgcgt atcgataagc ttagatcgag cacaagagga agagagagac
     8041 cctcactgct ggggagtccc tgccacactc agtcccccac cacactgaat ctcccctcct
     8101 cacagttgcc atgtagaccc cttgaagagg ggaggggcct agggagccgc accttgtcat
     8161 gtaccatcaa taaagtaccc tgtgctcaac cagttacttg tcctgtctta ttctagggtc
     8221 tggggcagag gggagggaag ctgggcttgt gtcaaggtga gacattcttg ctggggaggg
     8281 acctggtatg ttctcctcag actgagggta gggcctccaa acagccttgc ttgcttcgag
     8341 aaccatttgc ttcccgctca gacgtcttga gtgctacagg aagctggcac cactacttca
     8401 gagaacaagg ccttttcctc tcctcgctcc agtcctaggc tatctgctgt tggccaaaca
     8461 tggaagaagc tattctgtgg gcagccccag ggaggctgac aggtggagga agtcagggct
     8521 cgcactgggc tctgacgctg actggttagt ggagctcagc ctggagctga gctgcagcgg
     8581 gcaattccag cttggcctcc gcagctgtga ggtcttgagc acgtgctcta ttgctttctg
     8641 tgccctcgtg tcttatctga ggacatcgtg gccagcccct aaggtcttca agcaggattc
     8701 atctaggtaa accaagtacc taaaaccatg cccaaggcgg taaggactat ataatgttta
     8761 aaaatcggta aaaatgccca cctcgcatag ttttgaggaa gatgaactga gatgtgtcag
     8821 ggtgacttat ttccatcatc gtccttaggg gaacttgggt aggggcaagg cgtgtagctg
     8881 ggacctaggt ccagacccct ggctctgcca ctgaacggct cagttgcttt gggcagttac
     8941 tcccgggcct cactttgcac gtgtgcttac ctagtggaga caaaagtaca tacctcggta
     9001 gagcgcgcac gcctgtaacc ccagcacttt gggaggccaa ggtgggtgta tcacctgagg
     9061 tcaggagttt gagaccagcc tggccaacat ggtgaaactc cgtctctact aaaattacaa
     9121 aaatcagcca ggcttcatgg cacatgccta tagtcccagc tacaggcatg ctgaagcagg
     9181 agaatcgctt gcaccccgga ggcagaggct gcagtgagct gagaccacac cactgcactc
     9241 cagcctaggc aacagagtat gagactccat ctcaaaaaaa aaaaaagtac ctacctcaga
     9301 gttcaaacta gtgaatatta ggaagtgctt gagacagtga caccaaagtg cacaataaat
     9361 actcgccagt ttcattatta ttaaagaatc catttgaatg tcagctcaac acagcctcct
     9421 ataccgaggc attgtgaacc gcatctcccc agcttctcca ggcttttcca agaatcaggg
     9481 acactgtagc ctgttggtct cagtgtatga cagacacgga ggaagcacat ctttagctga
     9541 tacttaaaca gagaccctga gcgcacatac acccgcgcac acatgcatgg agcttcacct
     9601 tctctgtcat tctgcagtga ccaggagagc aagagctccc acctcccttc aaaacactgt
     9661 gcccatcccg ggcactaagg cctctttaaa gcacggcacc tccacgaggg agggccacag
     9721 ccacatacac tccacctggc aggtggacag cgtgagcacg tggaccatag cagggacaag
     9781 gtgccccggc cagccccaac gccctctgcc gctgacaggg acagaagccc tctccagctg
     9841 cgtgtgctgc agaggccatg cgtagcctcc agctgcattc tattccactc cagtgcctgg
     9901 gccagttagc accagtgtgg aagacagtga gctggctccg gacaacaggg atggaggaaa
     9961 ggtcccacat tcacattcct gatacgtgga caaggtgagg ggccgcaatc gctctggcag
    10021 cattttaaag atggggaagt agcagacacc cacgcgtgaa ggcaggagag ccccaactgt
    10081 ggtggaaatg gccccagaat ggtagggcca agcctagctc cagacacccc agagccctgg
    10141 agaagccaag actgagggag aaagcctgag ggaggagcgc cccagtcccc agggaccggc
    10201 ctggtgcaga gctgcagctg atgttcccct ctgtgcagcc ccaccctctg cctcgctgag
    10261 ctccctgctg cgagggcctc gggtgcaagg gggaggcagg tctctatctc atggagctgt
    10321 cagatgagac atcgcgatcg gagtcctcag cctcgcttgg cggcggcggc gggtcgctaa
    10381 gcgggaccgc agtgaaagca ggagactttc tagaaaaaaa caccagttgt caaccttggg
    10441 gcaggcagga atcctgaaga cggacggcac tcctcctcct gctgcctcac cctctggcag
    10501 cccgtgagaa gtaccggaag cgagggcggg gccgcgggat ggcgagggag cggcagggac
    10561 tgaactctct ccaaacccac cctgacaggg aaatgggccc cgcctgtgtc ttgggaactc
    10621 agaggctgag gtcaggcatg atggctcacg cctgtaattc cagcactttg ggaggcagag
    10681 gcgggtggat cacgacgtca ggagttcaag gcaagcctgg ccaacatggt gaaaccccat
    10741 ctctactaaa aatgcaaaaa ttagccaggt gtggtggcgg gcgccttgga ggctgaggca
    10801 agataatcac ttgaacctgg gaggcggagg ttgtagtgag ccaaaaaaaa aaaaaaaaga
    10861 aatagctgaa gtcacagtag gagagaagct gctgagcctc cagcaccctg actctagggc
    10921 cttggcttta tgtctatctg cagtattttt gtgattttta aaaattcact ttcttgttgc
    10981 ggtgtaactt acacagggtc aaatgcacaa atcatggccc tgactttaga taaaaatctg
    11041 cccccacaac ccttctgttc cttgccagtt tttaaactgc ctctaaccag gggaaccacc
    11101 agagctggtg gccttgggag gtttcagccc tcccgtcatg aatggacata gctcatccaa
    11161 ctgccaaggg agagagctgt gggtctgggc cagccccacc aggtaactcc caaagggcag
    11221 ccccacagca agatgtgacc cagtcattgc ctgagggtct ctggggctgt gttccaacct
    11281 ttctccccgc tgtgtccccc tggaaggccc catgcccagg ggaggcgcct accggtctcc
    11341 agactgggtg atgagccggc ggcaggtctc catctgcacg tccaggccgc gcttcatgct
    11401 gcacatctcc atgtactcgt gcaggtgccg gttcatgtcg ttcttggccg tggccaactc
    11461 cagctgtggt ggggcaggca gggccatcgg ggttaacagg tggcgttcac agcgcctctg
    11521 ttgcccccgc caggaggcca acacgccaag agcagtggct gggccggggg cccaggcagc
    11581 cattactgaa ggctcagatt ttaaaataaa ccagaccaag gcagaaggca gcgaatacac
    11641 cattgtttcc tctgcttttc ctggaaatct ttgcctatgg aggaatgcaa aggtactgct
    11701 gcaggctgtg ttcttttaag actgataaga gtggtgagga aagtcagtgt gggcgggatg
    11761 gagcagatcc acaggctccc agggccacac aggttcctat ggggcccggc tcacccctca
    11821 tcccctgtac tgcgctggtc ctgggctgtc ccctcactgc actgtgagct cccgtaaggg
    11881 ttagcgacag gttttcctca tcctggcaga cccagacctc gaagggaatt aactctctct
    11941 gtgacccgtg gcatacaagc ctggctaact gctgaaaggg gcccaatcct cttaccattc
    12001 tctccttatc actttatttt ctcacctaaa agccattttc agacactaaa aggaagactc
    12061 ccatattgga ggtgccattg attctgagac ctgtcctgat ttcagaaatg tcacacgtgg
    12121 aaaacacggg gctcagaatc aaagaaatcc ggtctccctc cccgggccca ttctgcagcc
    12181 cacactctga ggcaggcgga gaggcagggc ccaggcagca caaactcagt gccagggaca
    12241 gtcgattaat taagcgatcg caggccgccc gcggtggagc tccaattcgc cctatagtga
    12301 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    12361 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    12421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
    12481 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
    12541 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
    12601 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
    12661 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
    12721 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
    12781 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
    12841 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
    12901 gccgcgctta atgcgccgct acagggcgcg tcag
//
