LOCUS       sequence-161771-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of PDA1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP95
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    745..778
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
       61 tagaggatcc cattggcaca aatgtggttt cctttcttat tgctgtatcc aacttttgtg
      121 aagctacagg tgcgacaaac ataaaaaagt tcttgccgtt aagaagctga agttgcttct
      181 cgctatacca gaggggatta ttgtcgggag tcatgatgcg acatttaccc ggttgagtaa
      241 ggaaggggtg gaggaatcag aagagagata aacaaacata cacgtcaaaa cagtatagtt
      301 caagtaacac atatagacat gtacacgcgc gattacgtgt gtacgtgcac atgttaatgt
      361 tatatacata tatagatata gatatataat tgcaataatg aactacaaat tattataccc
      421 ccgttctttt ttttttcttt tttttagtaa tttttcttta gatttatcgg aatacttact
      481 ttggtgcttc accttcggcg gcagcaggtg gctgctgttg ctgatgttgt tgttgttgtt
      541 gttggtcttc agcttgaccg gactcggaca tgtctgaagt ccataaggtt aaattgtccc
      601 ttagcagttg catgataagt gtgctatctt tgtatgattc ttcagacaga gtgtccaact
      661 cagcaatagc gtcgtcaaaa gcttgcttgg ccaaatggca ggctttgtct ggagagtttt
      721 gaatttcgtc gagcaaccct taatataact tcgtataatg tatgctatac gaagttatta
      781 ggtctagaga tcccaataca acagatcacg tgatcttttt gtaagatgaa gttgaagtag
      841 tgttgcaccg tgccaatgca ggtggctatt agattaaata tgtgatttgt tctattaagt
      901 ttcctgtata attaatgggg agcgctgatt ctcttttggt acgcttccca tccagcattt
      961 ctgtatcttt caccttcaac cttaggatct ctacccttgg cgaaaagtcc tctgccaaca
     1021 atgatgat
//
