LOCUS       sequence-161773-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of PDA1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP95
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(222..932)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 catttaaatc attacgaccg agattcccgg gtaataactg atataattaa attgaagctc
       61 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
      121 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
      181 catcccttcc ctttgcaaat agtcctacta gtttaagatc ttttgtacaa ttcatccata
      241 ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc tctcttttcg
      301 tttggatctt tggataaggc agattgagtg gataagtaat ggttgtctgg taacaagact
      361 ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac agaaccatct
      421 tcaatgttgt gtctaatttt gaagttaact ttgataccat tcttttgttt gtcagccatg
      481 atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat gttaccatct
      541 tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc ttcaaacttg
      601 acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct ttcttgaaca
      661 taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc tgggtatctc
      721 gcaaaacatt gaacaccata accgaaagta gtgactaagg ttggccatgg aactggcaat
      781 ttaccagtag tacaaataaa ttttaaggtc aatttaccgt aagtagcatc accttcacct
      841 tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac caaaattggg
      901 acaacaccag tgaataattc ttcaccttta gaggatccca ttggcacaaa tgtggtttcc
      961 tttcttattg ctgtatccaa cttttgtgaa gctacaggtg c
//
