LOCUS       sequence-161775-       10995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-LRRTM2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-LRRTM2
FEATURES             Location/Qualifiers
     source          1..10995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3097..3106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4147..4188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5687..6275
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5740..5760)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6158..6169)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6278..6294)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6279..6295)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6800..6980
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7006..7023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7012..7236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7282..7710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7369..7388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7579..7600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7719..7739)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7724..8053
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7904..8039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7966..7985
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8101..8148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8167..8541
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8671..8792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8708..8727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8762..8781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8841..8857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8841..8857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8854..8876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8865..8881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8889..8895,8896..8913,8914..8919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8934..8955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9072..9089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9243..9831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9323..9342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10002..10793,10794..10862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10625..10644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10863..10967)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagcacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagag gatcccgcca ccatgggctt acatttcaag
     3121 tggccattag gggcccctat gctggcagca atatatgcaa tgagtatggt tttaaaaatg
     3181 ctgcctgccc tgggtatggc ggctagccag cttactccaa ctttctacga taactcttgt
     3241 cctaacgtga gcaacatcgt gcgagatact atcgtgaacg agttgcggtc tgatccgagg
     3301 atagccgctt caattttgcg gctgcatttc cacgattgtt ttgtaaacgg ctgtgatgct
     3361 tccatcctcc tcgacaatac cacctctttc aggactgaaa aggatgcctt cggtaacgcg
     3421 aatagtgcta gagggtttcc cgtgatcgat agaatgaaag ccgctgtgga gagcgcctgc
     3481 ccccggacag ttagttgtgc agaccttctg accatagcgg cccagcaatc cgtgacgctg
     3541 gccggtggcc ccagctggag ggtccccctg ggaaggaggg actctctgca ggcttttctg
     3601 gacttggcaa acgcaaacct gcctgctccc ttcttcaccc ttccccagct gaaggattcc
     3661 ttcaggaatg tcggcctgaa ccgaagtagt gatttggtgg ctctgtccgg aggccacaca
     3721 ttcggcaaga atcagtgcag gttcatcatg gacagactgt ataacttttc caacaccggc
     3781 ttgcctgacc ctaccctgaa cacaacatac ttgcagactc tcagagggct gtgtccgctc
     3841 aatggcaact tgtctgcact ggttgacttt gacctgcgca ccccaaccat cttcgacaat
     3901 aagtattacg tcaatctgga ggaacagaag ggcctgattc agtccgacca ggagctgttc
     3961 agttccccaa atgctaccga cactattcct cttgtgcgca gcttcgctaa tagcacacaa
     4021 acctttttca atgctttcgt ggaagctatg gatcggatgg gaaatataac gcccctgacg
     4081 ggtacgcagg gacagataag actgaactgt agggtcgtga acagcaactc tggctcgggc
     4141 tcgggcggca agcccatccc caaccccctg ctgggcctgg acagcaccac cggttgtcca
     4201 cccaaatgcc gctgcgagaa gctgctcttc tactgcgact ctcagggctt ccactcagtg
     4261 ccaaacgcca cagacaaggg ctctctgggc ctgtccctga ggcacaatca catcacagag
     4321 ctcgaaagag atcaatttgc cagcttcagt caacttactt ggctccactt agatcacaat
     4381 caaatttcaa cagtaaaaga agatgctttt caaggactat ataaacttaa ggaattaatc
     4441 ttaagttcca acaaaatatt ttacttgcca aacacaactt ttacccaact gattaacctg
     4501 caaaatttgg acctgtcttt taatcagctg tcatctctgc acccagagct cttctatggc
     4561 cttcggaagc tgcagacctt gcatttacgt tccaactccc tgcggactat cccagtacgc
     4621 ctgttctggg actgtcgtag tctggagttt ctggatttga gcacaaatcg tttgcgaagt
     4681 ttggctcgca atggatttgc aggattaatt aaactgagag agcttcacct agagcacaac
     4741 cagctgacga agattaattt tgctcatttc ctacggctaa gcagtctgca cacgctcttc
     4801 ttacaatgga acaaaatcag caacttgaca tgtgggatgg agtggacctg gggcacttta
     4861 gaaaagctag acctgactgg aaatgaaatc aaagccatcg acttgacagt gtttgaaacg
     4921 atgcccaatc ttaaaatact cctcatggat aacaacaagt taaacagcct tgattccaag
     4981 atcttaaact ccctgagatc cctcacaacc gttggtctct ctggcaatct gtgggaatgc
     5041 agcgcccgaa tatgtgctct ggcctcctgg ctgggcagtt tccaaggtcg gtgggaacac
     5101 tccatcctat gccacagtcc tgaccacacc caaggagagg atattctaga tgcagtccat
     5161 ggatttcagc tctgctggaa tttgtcaacc actgtcactg tcatggctac aacttataga
     5221 gatccaacca ctgaatatac aaaaagaata agctcatcaa gttaccatgt gggagacaaa
     5281 gaaatcccaa ctactgcagg catagcagtt actaccgagg aacactttcc tgaaccagac
     5341 aatgccatct tcactcagcg ggtaattacg ggaacaatgg ctttattgtt ttctttcttt
     5401 tttattattt ttatagtgtt catctccagg aagtgctgcc ctcccacttt aagaagaatt
     5461 aggcagtgct caatggttca gaaccacagg cagctccgat cccaaacacg actccatatg
     5521 tcaaacatgt cagaccaagg accgtataat gaatatgaac ccacccatga aggacccttc
     5581 atcatcatta atggttatgg acagtgcaag tgtcagcagc tgccatacaa agaatgtgaa
     5641 gtataaggcg cgcccctgca gggaattcga tatcaagctt atcgataatc aacctctgga
     5701 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5761 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5821 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5881 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5941 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6001 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6061 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6121 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6181 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6241 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     6301 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6361 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6421 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6481 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6541 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     6601 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     6661 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6721 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     6781 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6961 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     7021 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     7081 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     7141 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     7201 atagcaggca tgctggggat gcggcgggct ctatggcttc tgaggcggaa agaaccagct
     7261 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7681 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     7741 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7801 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7861 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7921 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7981 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8041 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     8101 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     8161 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     8221 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     8281 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     8341 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     8401 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     8461 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     8521 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8641 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8821 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8881 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8941 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9001 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9061 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10981 aaagtgccac ctgac
//
