LOCUS       sequence-161778-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of CYC1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP97
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    394..427
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaccattaac atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt
       61 cacctttaga ggatcccatt attaatttag tgtgtgtatt tgtgtttgtg tgtctataga
      121 agtatagtaa tttatgctgc aaaggtccta atgtataagg aaagaatatt tagagaaaag
      181 aagaaaacaa gagttttata tacatacaga gcacatgcat gccatatgat catgtgttgt
      241 cgcacacata tatatatgcc tgtatgtgtc agcactaaag ttgcctggcc atccacgcta
      301 tatatacacg cctggcggat ctgctcgagg attgcctacg cgtgggcttg atccaccaac
      361 caacgctcgc caatgagtcg agcaaccctt aatataactt cgtataatgt atgctatacg
      421 aagttattag gtctagagat cccaatacaa cagatcacgt gatctttttg taagatgaag
      481 ttgaagtagt gttgcaccgt gccaatgcag gtggctatta gattaaatat gtgatttgtt
      541 ctattaagtt tcctgtataa ttaatgggga gcgctgattc tcttttggta cgcttcccat
      601 ccagcatttc tgtatctttc accttcaacc ttaggatctc tacccttggc gaaaagtcct
      661 ctgccaacaa tgatgatatc tgatccacca cttacaactt cgtcgacggt tctgtactgc
      721 tgacccaatg catcgccttt gtcgtctaaa cctacacctg gggtcatgat tagccaatca
      781 aacccttctt ctcttcctcc catatcgttc tgagcaatga acccaataac gaaatcttta
      841 tcactctttg caatatcaac ggtaccctta gtatattcac cgtgtgctag agaacccttg
      901 gaagacaatt cagcaagcat caataatccc cttggttctt tggtgacctc ttgcgcacct
      961 tgtttcaagc cagcaacaat accagcacca gtaaccccgt gggcgttggt gatatcagac
     1021 cattctgcga tacggtaaac gcccgatg
//
