LOCUS       sequence-161788-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of ADH2 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPH7
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    563..596
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccattgtgta ttacgatata gttaatagtt gatagttgat tgtatgcttt
      121 ttgtagcttg atattctatt taccaagaag aaacaagaag tgataaaaac aacaagagag
      181 cagtagtaag agtatttcga gtgtgaaaaa agtcgctact ggcactctat ttatatgtga
      241 taggcatgct atagctttac caaaaagtga accccatttt tatgctctcc tctgcctttt
      301 tttttttttt ttcattctct caatctgaaa ttctcttatt tctccaactt ataagttgga
      361 gatgcccggt gttccggcag aggagatcag tctcgtgaag tggatggttt cccgcctgcg
      421 ggcaaaacgt cataacattt ttatgagcga aagccgttaa tgaagacaaa atcccttaat
      481 taaaacatta gaatggtgat tagaaaggca ggattaatca gttacacagg ctgtaaccgg
      541 agagagtcga gcaaccctta atataacttc gtataatgta tgctatacga agttattagg
      601 tctagagatc ccaatacaac agatcacgtg atctttttgt aagatgaagt tgaagtagtg
      661 ttgcaccgtg ccaatgcagg tggctattag attaaatatg tgatttgttc tattaagttt
      721 cctgtataat taatggggag cgctgattct cttttggtac gcttcccatc cagcatttct
      781 gtatctttca ccttcaacct taggatctct acccttggcg aaaagtcctc tgccaacaat
      841 gatgatatct gatccaccac ttacaacttc gtcgacggtt ctgtactgct gacccaatgc
      901 atcgcctttg tcgtctaaac ctacacctgg ggtcatgatt agccaatcaa acccttcttc
      961 tcttcctccc atatcgttct ga
//
