LOCUS       sequence-161795-        3993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GPR137B, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GPR137B_p.Y135N
FEATURES             Location/Qualifiers
     source          1..3993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1872..1971)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1988..2007)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1989..2007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2012..2028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2012..2028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2459..3250
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3343..3931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3832..3851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaggcccg agcgtccccg gccgcgcggc agcgcccccg gcccgatgga
      721 gaccccgccg tgggacccag cccgcaacga ctcgctgccg cccacgctga ccccggccgt
      781 gcccccctac gtgaagcttg gcctcaccgt cgtctacacc gtgttctacg cgctgctctt
      841 cgtgttcatc tacgtgcagc tctggctggt gctgcgttac cgccacaagc ggctcagcta
      901 ccagagcgtc ttcctctttc tctgcctctt ctgggcctcc ctgcggaccg tcctcttctc
      961 cttctacttc aaagacttcg tggcggccaa ttcgctcagc cccttcgtct tctggctgct
     1021 ctactgcttc cctgtgtgcc tgcagttttt caccctcacg ctgatgaact tgaacttcac
     1081 gcaggtgatt ttcaaagcca agtcaaaata ttctccagaa ttactcaaat accggttgcc
     1141 cctctacctg gcctccctct tcatcagcct tgttttcctg ttggtgaatt taacctgtgc
     1201 tgtgctggta aagacgggaa attgggagag gaaggttatc gtctctgtgc gagtggccat
     1261 taatgacacg ctcttcgtgc tgtgtgccgt ctctctctcc atctgtctct acaaaatctc
     1321 taagatgtcc ttagccaaca tttacttgga gtccaagggc tcctccgtgt gtcaagtgac
     1381 tgccatcggt gtcaccgtga tactgcttta cacctctcgg gcctgctaca acctgttcat
     1441 cctgtcattt tctcagaaca agagcgtcca ttcctttgat tatgactggt acaatgtatc
     1501 agaccaggca gatttgaaga atcagctggg agatgctgga tacgtattat ttggagtggt
     1561 gttatttgtt tgggaactct tacctaccac cttagtcgtt tatttcttcc gagttagaaa
     1621 tcctacaaag gaccttacca accctggaat ggtccccagc catggattca gtcccagatc
     1681 ttatttcttt gacaaccctc gaagatatga cagtgatgat gaccttgcct ggaacattgc
     1741 ccctcaggga cttcagggag gttttgctcc agattactat gattggggac aacaaactaa
     1801 cagcttcctg gcacaagcag gaactttgca agactcaact ttggatcctg acaaaccaag
     1861 ccttgggtga ttgccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1921 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1981 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2041 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2101 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2161 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2221 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2281 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2341 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2401 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2461 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2521 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2581 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2641 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2701 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2761 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2821 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2881 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2941 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3001 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3061 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3121 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3181 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3241 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3481 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3961 cctggccttt tgctggcctt ttgctcacat gtt
//
