LOCUS       sequence-161796-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPB5A
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(86..527)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(92..115)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    410..527
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    760..793
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccattatagt tttttctcct tgacgttaaa gtatagaggt atattaacaa
      121 ttttttgttg atacttttat gacatttgaa taagaagtaa tacaaaccga aaatgttgaa
      181 agtattagtt aaagtggtta tgcagctttt gcatttatat atctgttaat agatcaaaaa
      241 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattattat
      301 cctatggttg ttaatttgat tcgttgattt gaaggtttgt ggggccaggt tactgccaat
      361 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
      421 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agaccaggac gcacggagga
      481 gagtcttccg tcggagggct gtcgcccgct cggcggcttc taatccgtac ttcaatatag
      541 caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt aggctaagat
      601 aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat atgtatatgg
      661 tggtattgcc atgtaatatg attattaaac ttctttgcgt ccatccaaaa aaaaagtaag
      721 aatttttgaa aattcaatat aagtcgagca acccttaata taacttcgta taatgtatgc
      781 tatacgaagt tattaggtct agagatccca atacaacaga tcacgtgatc tttttgtaag
      841 atgaagttga agtagtgttg caccgtgcca atgcaggtgg ctattagatt aaatatgtga
      901 tttgttctat taagtttcct gtataattaa tggggagcgc tgattctctt ttggtacgct
      961 tcccatccag catttctgta tctttcacct tcaaccttag gatctc
//
