LOCUS       sequence-161798-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPB5A
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(221..931)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(958..981)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 atttaaatca ttacgaccga gattcccggg taataactga tataattaaa ttgaagctct
       61 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
      121 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
      181 atcccttccc tttgcaaata gtcctactag tttaagatct tttgtacaat tcatccatac
      241 catgggtaat accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt
      301 ttggatcttt ggataaggca gattgagtgg ataagtaatg gttgtctggt aacaagactg
      361 gaccatcacc aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt
      421 caatgttgtg tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga
      481 tgtaaacatt gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt
      541 ctttaaaatc aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga
      601 cttcagctct ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat
      661 aaccttctgg catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctcg
      721 caaaacattg aacaccataa ccgaaagtag tgactaaggt tggccatgga actggcaatt
      781 taccagtagt acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt
      841 caccggagac agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga
      901 caacaccagt gaataattct tcacctttag aggatcccat tatagttttt tctccttgac
      961 gttaaagtat agaggtatat taacaatttt ttgttgatac ttttatgaca tttgaataag
     1021 aagtaataca aaccga
//
