LOCUS       sequence-161799-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL10 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pILGFPB6
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        290..731
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    290..407
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     702..725
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    760..793
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccatttatat tgaattttca aaaattctta cttttttttt ggatggacgc
      121 aaagaagttt aataatcata ttacatggca ataccaccat atacatatcc atatctaatc
      181 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      241 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      301 ccgccgagcg ggcgacagcc ctccgacgga agactctcct ccgtgcgtcc tggtcttcac
      361 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      421 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      481 accttcaaat caacgaatca aattaacaac cataggataa taatgcgatt agttttttag
      541 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      601 aaatgcaaaa gctgcataac cactttaact aatactttca acattttcgg tttgtattac
      661 ttcttattca aatgtcataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      721 acgtcaagga gaaaaaacta tagtcgagca acccttaata taacttcgta taatgtatgc
      781 tatacgaagt tattaggtct agagatccca atacaacaga tcacgtgatc tttttgtaag
      841 atgaagttga agtagtgttg caccgtgcca atgcaggtgg ctattagatt aaatatgtga
      901 tttgttctat taagtttcct gtataattaa tggggagcgc tgattctctt ttggtacgct
      961 tcccatccag catttctgta tctttcacct tcaaccttag gatctctacc ct
//
