LOCUS       sequence-161802-       11007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-LRRTM1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-LRRTM1
FEATURES             Location/Qualifiers
     source          1..11007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4144..4185
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5699..6287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5752..5772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6170..6181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6290..6306)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6291..6307)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6812..6992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7018..7035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7024..7248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7294..7722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7381..7400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7591..7612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7731..7751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7736..8065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7916..8051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7978..7997
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8113..8160
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8179..8553
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8683..8804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8720..8739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8774..8793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8853..8869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8853..8869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8866..8888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8877..8893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8901..8907,8908..8925,8926..8931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8946..8967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9084..9101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9255..9843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9335..9354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10014..10805,10806..10874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10637..10656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10875..10979)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagcacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagac gccaccatgg atttcctgct gctcggtctc
     3121 tgtctatact ggctgctgag gaggccctcg ggggtggtct tgtgtctgct gggggcctgc
     3181 tttcagatgc tgcccgccgc tagccagctt actccaactt tctacgataa ctcttgtcct
     3241 aacgtgagca acatcgtgcg agatactatc gtgaacgagt tgcggtctga tccgaggata
     3301 gccgcttcaa ttttgcggct gcatttccac gattgttttg taaacggctg tgatgcttcc
     3361 atcctcctcg acaataccac ctctttcagg actgaaaagg atgccttcgg taacgcgaat
     3421 agtgctagag ggtttcccgt gatcgataga atgaaagccg ctgtggagag cgcctgcccc
     3481 cggacagtta gttgtgcaga ccttctgacc atagcggccc agcaatccgt gacgctggcc
     3541 ggtggcccca gctggagggt ccccctggga aggagggact ctctgcaggc ttttctggac
     3601 ttggcaaacg caaacctgcc tgctcccttc ttcacccttc cccagctgaa ggattccttc
     3661 aggaatgtcg gcctgaaccg aagtagtgat ttggtggctc tgtccggagg ccacacattc
     3721 ggcaagaatc agtgcaggtt catcatggac agactgtata acttttccaa caccggcttg
     3781 cctgacccta ccctgaacac aacatacttg cagactctca gagggctgtg tccgctcaat
     3841 ggcaacttgt ctgcactggt tgactttgac ctgcgcaccc caaccatctt cgacaataag
     3901 tattacgtca atctggagga acagaagggc ctgattcagt ccgaccagga gctgttcagt
     3961 tccccaaatg ctaccgacac tattcctctt gtgcgcagct tcgctaatag cacacaaacc
     4021 tttttcaatg ctttcgtgga agctatggat cggatgggaa atataacgcc cctgacgggt
     4081 acgcagggac agataagact gaactgtagg gtcgtgaaca gcaactctgg ctcgggctcg
     4141 ggcggtaagc ccatccccaa ccccctgctg ggcctggaca gcaccaccgg tgcccccagc
     4201 gggtgcccgc agctgtgccg gtgcgagggg cggctgctgt actgcgaggc gctcaacctc
     4261 accgaggcgc cccacaacct gtccggcctg ctgggcttgt ccctgcgcta caacagcctc
     4321 tcggagctgc gcgccggcca gttcacgggg ttaatgcagc tcacgtggct ctatctggat
     4381 cacaatcaca tctgctccgt gcagggggac gcctttcaga aactgcgccg agttaaggaa
     4441 ctcacgctga gttccaacca gatcacccaa ctgcccaaca ccaccttccg gcccatgccc
     4501 aacctgcgca gcgtggacct ctcgtacaac aagctgcagg cgctcgcgcc cgacctcttc
     4561 cacgggctgc ggaagctcac cacgctgcat atgcgggcca acgccatcca gtttgtgccc
     4621 gtgcgcatct tccaggactg ccgcagcctc aagtttctcg acatcggata caatcagctc
     4681 aagagtctgg cgcgcaactc tttcgccggc ttgtttaagc tcaccgagct gcacctcgag
     4741 cacaacgact tggtcaaggt gaacttcgcc cacttcccgc gcctcatctc cctgcactcg
     4801 ctctgcctgc ggaggaacaa ggtggccatt gtggtcagct cgctggactg ggtttggaac
     4861 ctggagaaaa tggacttgtc gggcaacgag atcgagtaca tggagcccca tgtgttcgag
     4921 accgtgccgc acctgcagtc cctgcagctg gactccaacc gcctcaccta catcgagccc
     4981 cggatcctca actcttggaa gtccctgaca agcatcaccc tggccgggaa cctgtgggat
     5041 tgcgggcgca acgtgtgtgc cctagcctcg tggctcaaca acttccaggg gcgctacgat
     5101 ggcaacttgc agtgcgccag cccggagtac gcacagggcg aggacgtcct ggacgccgtg
     5161 tacgccttcc acctgtgcga ggatggggcc gagcccacca gcggccacct gctctcggcc
     5221 gtcaccaacc gcagtgatct ggggccccct gccagctcgg ccaccacgct cgcggacggc
     5281 ggggaggggc agcacgacgg cacattcgag cctgccaccg tggctcttcc aggcggcgag
     5341 cacgccgaga acgccgtgca gatccacaag gtggtcacgg gcaccatggc cctcatcttc
     5401 tccttcctca tcgtggtcct ggtgctctac gtgtcctgga agtgtttccc agccagcctc
     5461 aggcagctca gacagtgctt tgtcacgcag cgcaggaagc aaaagcagaa acagaccatg
     5521 catcagatgg ctgccatgtc tgcccaggaa tactacgttg attacaaacc gaaccacatt
     5581 gagggagccc tggtgatcat caacgagtat ggctcgtgta cctgccacca gcagcccgcg
     5641 agggaatgcg aggtgtaagg cgcgcccctg cagggaattc gatatcaagc ttatcgataa
     5701 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5761 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5821 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5881 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5941 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6001 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6061 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6121 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6181 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6241 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6301 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6361 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6421 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6481 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     6541 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6601 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6661 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6721 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     6781 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     6841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6961 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7021 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7081 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7141 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7201 attgggaaga caatagcagg catgctgggg atgcggcggg ctctatggct tctgaggcgg
     7261 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7321 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7381 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7441 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7501 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7561 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7621 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7681 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7741 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7801 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7861 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7921 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7981 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8041 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8101 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     8161 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     8221 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     8281 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     8341 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     8401 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     8461 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     8521 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     8581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8821 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10981 acatttcccc gaaaagtgcc acctgac
//
