LOCUS       sequence-161804-        4068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZNF597, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ZNF597_p.L283V
FEATURES             Location/Qualifiers
     source          1..4068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1947..2046)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2063..2082)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2064..2082)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2087..2103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2087..2103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2534..3325
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3418..4006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3907..3926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcgtcca tgcccccgac gcccgaggcc cagggaccaa tactgtttga
      721 ggatctggct gtgtattttt ctcaagagga gtgcgtgact ctgcaccctg cccagaggtc
      781 cctcagcaaa gatggtacaa aagagtcttt ggaggatgcg gctttgatgg gagaggaagg
      841 caagcctgag attaatcagc agttaagcct agagtctatg gaacttgacg agcttgcctt
      901 agaaaagtac cccattgctg caccccttgt cccttaccca gaaaaatcct ctgaggatgg
      961 agttggaaac cctgaagcga aaatattaag tggaactccc acttacaaga gaagggtcat
     1021 cagcctttta gttaccattg aaaaccacac cccattagta gaactctctg aatatttagg
     1081 aaccaacaca ctttctgaaa ttcttgattc tccctgggaa ggagccaaaa atgtgtacaa
     1141 atgtcctgag tgtgaccaaa acttcagcga tcattcatac ctagttttgc atcagaaaat
     1201 tcattcagga gagaaaaaac ataaatgtgg tgactgtgga aagatcttca atcatagagc
     1261 caacctgagg acacacagga gaatccatac tggtgagaaa ccttataagt gtgccaagtg
     1321 cagtgccagc tttcgccagc actctcatct atcccgacac atgaatagcc acgtaaagga
     1381 gaagccctat acatgtagca tatgtggtag aggttttatg tggctcccag gattggcaca
     1441 gcatcagaaa agccacagtg ctgaaaacac ctacgaatct actaactgtg ataaacattt
     1501 taatgagaaa ccaaatgttg ctttgcctga ggaaacattc gtatcaggcc cccagtacca
     1561 gcacactaag tgcatgaaga gcttcaggca gtccttatat cctgcccttt ccgagaagag
     1621 ccacgacgag gactctgaac gctgcagcga tgatggggac aatttcttct cattctcaaa
     1681 attcaagccc ttacagtgtc ctgactgtga catgaccttt ccttgtttct ctgagcttat
     1741 ttcccatcag aacattcata cagaggaaag gccccataag tgcaaaacat gcgaggaaag
     1801 ttttgctttg gactcagaac ttgcatgcca ccagaagagc cacatgctag cggaaccttt
     1861 taaatgtacc gtgtgtggga aaactttcaa gtcgaatttg catctcatta ctcataagcg
     1921 aactcacata aaaaacacca cgtgattgcc aactttcttg tacaaagttg gcattataag
     1981 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2041 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2101 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2161 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2221 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2281 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2341 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2401 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2461 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2521 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2581 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2641 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     2701 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2761 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2821 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2881 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2941 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3001 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3061 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3121 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3181 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3241 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3301 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3361 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
