LOCUS       sequence-161806-       10037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. 
            coli with TEV-cleavable N-terminal His-MBP tag on Cas5c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
FEATURES             Location/Qualifiers
     source          1..10037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    46..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             85..107
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             126..143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             150..1247
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1104..1113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1221..1244
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     protein_bind    1266..1290
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1311..1331
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(4751..4775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4789..4806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4859..4877)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4873..4920
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4957..5412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5044..5063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5254..5275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5438..5542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5543..5611,5612..6403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5761..5780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6574..7162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7063..7082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7316..7333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7348..7487
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7413..7432)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7573..7595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7589..7780)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8589..9671)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9633..9652
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9672..9749)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9955..9974
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agatccagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
       61 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      121 gcagccatca tcatcatcat cacggtacca aaactgaaga aggtaaactg gtaatctgga
      181 ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata
      241 ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg
      301 cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg
      361 ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc
      421 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg
      481 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag
      541 agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc
      601 tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt
      661 atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg
      721 gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact
      781 ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg
      841 catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca
      901 agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc
      961 cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg
     1021 aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt
     1081 tggcgaaaga tccacgtatt gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc
     1141 cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg atcaacgccg
     1201 ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactggt accggatctt
     1261 acatcacaag tttgtacaaa aaagcaggct ccgcggccgc cgtactcgag gaaaacctgt
     1321 attttcaggg ctccttcacc atgacacatg gggctgttaa gacatacggc atcagactgc
     1381 gggtatgggg cgactacgcc tgcttcacgc ggccggagat gaaggtggaa cgggtgtcat
     1441 acgatgtcat gcccccttcc gccgcacggg gcatccttga ggccatccac tggaagccgg
     1501 ccatcaggtg gattgtggac aggattcatg tgctgcggcc catcgtcttc gacaacgtgc
     1561 ggcgcaacga ggtcagttcg aagataccca agcccaatcc ggcaacggcc atgcgcgaca
     1621 ggaagccgct ctactttctg gtcgatgacg gcagcaaccg ccagcaacgg gcggcgaccc
     1681 tgctgcgcaa cgtcgactat gtcatcgaag cccacttcga actgacagac aaggccggag
     1741 cggaagacaa cgcgggcaag catctggaca tcttcaggcg tcgtgcgcgc gccgggcagt
     1801 cgttccagca gccatgcctc ggctgccgcg agtttccggc gtcgttcgaa ctgctggagg
     1861 gggatgtgcc gctctcgtgc tacgcgggcg agaagcgcga ccttggctac atgctgctcg
     1921 acatcgactt cgaacgcgac atgacgccgc tgttcttcaa ggccgtcatg gaagacggag
     1981 tgataacgcc cccgtcgcgc acgtcaccgg aggtgagggc atgatcctgc aggcattgca
     2041 tggctattac cagcgcatga gcgcagaccc cgatgcgggg atgccgccct acgggaccag
     2101 catggagaac atctcgttcg cgctggtgct ggatgcgaaa gggacgctac ggggcatcga
     2161 ggacttgcgg gaacaggaag gcaagaagct gcggccccgc aagatgctgg ttcctatcgc
     2221 ggaaaagaag gggaacggca tcaaaccgaa cttcctgtgg gagaacacca gttatattct
     2281 gggggtggac gccaagggca agcaggaacg tacagacaag tgccacgccg cgttcattgc
     2341 ccacatcaag gcctactgtg acactgcaga ccaagacctt gccgccgttc tgcagttcct
     2401 tgaacacggc gaaaaagacc tctccgcttt tccggtttcg gaagaggtca tcggctccaa
     2461 tatcgtcttt cgcatcgagg gcgaaccggg cttcgtccat gagcgtccgg ctgcgcggca
     2521 ggcttgggcc aactgcctga accggcggga gcaagggcta tgtggtcaat gcctcatcac
     2581 cggggagcgc cagaagccga ttgcccaact ccatccgtcg atcaaaggcg ggcgagacgg
     2641 cgttcgcggt gcacaggctg ttgcctcgat agtgtccttt aacaacaccg cctttgaatc
     2701 ttacgggaaa gaacagtcga tcaatgcacc cgtcagtcaa gaagcggcat tctcttatgt
     2761 cacagccctg aattacctgc tcaacccgtc aaatcgacag aaggtcacca tcgctgacgc
     2821 caccgtggtc ttctgggccg aacgcagcag cccggcagag gacatctttg caggcatgtt
     2881 cgacccgcct tccaccacgg cgaaaccgga gtcctcgaac gggacgccgc cggaagacag
     2941 tgaagaaggc agccagcctg acacagccag agacgacccc catgccgctg ccagaatgca
     3001 cgacctgctt gtggccatcc gttccggcaa gcgcgccacg gacatcatgc ccgacatgga
     3061 cgagtccgtg cgcttccatg tgcttggcct gtcgcccaac gccgcccgcc tgtccgtccg
     3121 cttctgggag gtcgataccg tcggtcacat gctggacaag gtggggcggc actaccgtga
     3181 actcgagatc attccgcaat tcaacaacga gcaggagttc ccgtctcttt caaccctgct
     3241 gcggcagact gccgtcttga acaagaccga gaacatctcg cctgtacttg caggcgggct
     3301 attcagggcc atgctcaccg gcgggcccta tccgcaaagc ctgttgcccg ccgtgcttgg
     3361 acgcatccgc gccgagcacg ccagaccaga agataaaagc cgctataggc ttgaagttgt
     3421 gacgtattat cgagccgcct tgatcaaggc gtatctcata agaaacagaa agctggaggt
     3481 gcccgtgtct ctcgaccccg caagaacaga ccgcccgtac ctgctcggac gactcttcgc
     3541 cgttctggaa aaagcgcagg aagatgccgt gcccggtgcg aacgccacca tcaaggaccg
     3601 ctatctggcc tccgcatcgg ccaatccggg gcaggtattc cacatgctgc tcaagaatgc
     3661 gtccaaccac acggccaaac tccgcaagga ccctgaaaga aaggggtcgg ccatccacta
     3721 cgaaatcatg atgcaagaga ttatcgacaa catcagcgat tttccggtga ccatgtcgtc
     3781 cgacgaacaa ggcctgttca tgatcggcta ctaccaccag cgcaaggccc ttttcaccaa
     3841 gaagaacaag gagaactagc catgaccgcc attgccaaca gatacgagtt cgtgctgctt
     3901 ttcgatgtgg agaacggcaa ccccaacggc gaccccgacg cgggcaacat gccgcgcatc
     3961 gaccccgaga ccgggcatgg tctggtcacc gacgtgtgcc tgaagcgcaa gatacgcaac
     4021 catgtggccc tgacaaaaga aggtgccgag cggttcaata tctatattca ggaaaaggcc
     4081 attctcaacg aaacccacga acgcgcctac acggcctgcg acctgaaacc cgaacccaag
     4141 aaactgccca agaaggtcga agacgccaag agggtcaccg actggatgtg cacgaacttc
     4201 tacgacatcc gcacgttcgg cgccgtcatg accacagagg tcaactgcgg gcaggtgcgc
     4261 ggccccgtgc agatggcctt tgccagaagt gtggaacccg tcgtgccgca agaggtgagc
     4321 atcacccgca tggcagtcac cacgaaggca gaggccgaaa agcagcaggg agacaaccgc
     4381 accatgggtc gcaagcacat cgtgccctac ggcctgtatg tggcgcacgg cttcatctcc
     4441 gcaccgctgg cagagaagac aggcttctcc gatgaagacc tgacgctgtt ctgggacgcc
     4501 ctcgtcaaca tgttcgagca cgaccgttcg gcggcacgcg gcctcatgag cagccgcaaa
     4561 ctcatcgtct tcaagcacca gaacaggctc ggcaacgccc ccgcccacaa gttgttcgac
     4621 ctcgtgaagg tcagccgtgc agagggttcg tccggccccg cccggtcgtt tgccgactat
     4681 gcggtgacag tggggcaggc ccccgaaggc gtagaggtca aagagatgct gtagaagggt
     4741 gggcgcgccg acccagcttt cttgtacaaa gtggtgatgt acctcgagca ccaccaccac
     4801 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     4861 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     4921 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     4981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5401 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5761 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6061 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     6121 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6181 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6241 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6301 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6361 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6421 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6481 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6721 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6961 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7201 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7261 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7321 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7381 cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7441 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     7501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7561 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7621 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     7681 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     7741 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     7801 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     7861 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     7921 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     7981 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     8041 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     8101 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     8161 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     8221 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct
     8281 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     8341 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     8401 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     8461 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     8521 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     8701 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
     8761 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     8821 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     8881 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     8941 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     9001 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     9061 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     9121 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     9181 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     9241 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     9301 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     9361 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     9421 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     9481 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     9541 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     9601 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     9661 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     9721 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
     9781 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
     9841 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     9901 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     9961 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
    10021 tccggcgtag aggatcg
//
