LOCUS       sequence-161807-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL2 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pILGFPQ3
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    787..820
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc tttagaggat
       61 cccattatga aagaattatt ttttttatta tgttaatctt gtgtttactt aactattgct
      121 attcttgatg ataattgaat aaggtgcata atgaagagca attcacaaca ccaaattttc
      181 aatccaatta ctgattgttt atatatgtct acaaaactaa tcctatctcc acattttagc
      241 ctgcgaaatg tttgtttttt aaacaatagc tctccagaac attgtataat ttaagaatat
      301 gtgcacagtt aactttctag caggagtata atgccatttg ctccccatct tgagatggga
      361 agggcttaac taatctcggt tcggagtgat ccgccccgat actgccttct gccttaatat
      421 cgtccaaggc acatggaccc ctgaacggcg cagatatctc cgcacggacg aaagaccgcc
      481 ggtgccttcc tgaggcaacc gcccctttcg aatatagatc acgtgaccca tttttagcta
      541 ctaatagaaa aagaaattgc aacctactta agccattccg gaaggaagct ttccgaattg
      601 acgttgaata ccttttctga aaagattcta ccgcatttaa ttggagttta atgcaaagta
      661 gttgccagtt acaagttgag tgcggctacc tgatgttttc cagtcctata ttgctgtgat
      721 ctgaatctgg aaagttcctc tggtccgtaa acctccttgg attagtacag tcgagcaacc
      781 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga gatcccaata
      841 caacagatca cgtgatcttt ttgtaagatg aagttgaagt agtgttgcac cgtgccaatg
      901 caggtggcta ttagattaaa tatgtgattt gttctattaa gtttcctgta taattaatgg
      961 ggagcgctga ttctcttttg gtacgcttcc catccagcat ttctgta
//
