LOCUS       sequence-161808-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL2 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pILGFPQ3
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(222..932)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 catttaaatc attacgaccg agattcccgg gtaataactg atataattaa attgaagctc
       61 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
      121 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
      181 catcccttcc ctttgcaaat agtcctacta gtttaagatc ttttgtacaa ttcatccata
      241 ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc tctcttttcg
      301 tttggatctt tggataaggc agattgagtg gataagtaat ggttgtctgg taacaagact
      361 ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac agaaccatct
      421 tcaatgttgt gtctaatttt gaagttaact ttgataccat tcttttgttt gtcagccatg
      481 atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat gttaccatct
      541 tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc ttcaaacttg
      601 acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct ttcttgaaca
      661 taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc tgggtatctc
      721 gcaaaacatt gaacaccata accgaaagta gtgactaagg ttggccatgg aactggcaat
      781 ttaccagtag tacaaataaa ttttaaggtc aatttaccgt aagtagcatc accttcacct
      841 tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac caaaattggg
      901 acaacaccag tgaataattc ttcaccttta gaggatccca ttatgaaaga attatttttt
      961 ttattatgtt aatcttgtgt ttacttaact attgctattc ttgatgataa ttga
//
