LOCUS       sequence-161811-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of 
            catalytic Cys2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: pET30a-SRAP-delCys2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..79
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             86..130
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             890..907
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tctggtgcca cgcggttctg gtatgaaaga aaccgctgct gctaaattcg aacgccagca
      121 catggacagc ccagatctgg ggcgaacgtc ctgtcacttg cccagagagg ttctcaccag
      181 ggcctgcgcc tatcaggatc ggcagggccg gcggcggctc ccgcagtgga gggaccccga
      241 caagtactgc ccctcctaca acaagagccc gcagtccagc agccccgtgc tgctctccag
      301 actgcacttt gagaaggatg cagactcatc agatcggata attattccca tgcgatgggg
      361 cttagtccca tcttggttca aagaaagtga tccttctaag ctgcagttca acactaccaa
      421 ctgtcgcagt gataccataa tggagaagca gtcattcaag gttcctctgg ggaaaggacg
      481 gcggtgtgtt gttttagcag atggattcta cgagtggcag cggtgtcagg gaacaaacca
      541 gaggcaacca tacttcatct attttcctca aatcaagaca gagaagtcag gtgggaacga
      601 tgcttcagac agctctgaca acaaggaaaa ggtctgggac aactggaggc tgctgacaat
      661 ggcagggatc tttgactgct gggaagcgcc agggggagag tgcctgtatt cctacagcat
      721 catcactgtg gattcctgca gaggtttgag tgacatccac agcaggatgc ctgccatact
      781 agatggagaa gaagcagtct ccaaatggct cgactttggt gaggtcgcca ctcaggaagc
      841 tctgaagcta atccacccca tagacaatat caccttccat ccactcgagc accaccacca
      901 ccaccactga natccggctg ctaaca
//
