LOCUS       sequence-161815-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of GAL7 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pILGFPQ4
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(219..929)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 ttaaatcatt acgaccgaga ttcccgggta ataactgata taattaaatt gaagctctaa
       61 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
      121 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
      181 cccttccctt tgcaaatagt cctactagtt taagatcttt tgtacaattc atccatacca
      241 tgggtaatac cagcagcagt aacaaattct aacaagacca tgtggtctct cttttcgttt
      301 ggatctttgg ataaggcaga ttgagtggat aagtaatggt tgtctggtaa caagactgga
      361 ccatcaccaa ttggagtatt ttgttgataa tggtcagcta attgaacaga accatcttca
      421 atgttgtgtc taattttgaa gttaactttg ataccattct tttgtttgtc agccatgatg
      481 taaacattgt gagagttata gttgtattcc aatttgtgac ctaaaatgtt accatcttct
      541 ttaaaatcaa taccttttaa ttcgattcta ttaactaagg tatcaccttc aaacttgact
      601 tcagctctgg tcttgtagtt accgtcatct ttgaaaaaaa tagttctttc ttgaacataa
      661 ccttctggca tggcagactt gaaaaagtca tgttgtttca tatgatctgg gtatctcgca
      721 aaacattgaa caccataacc gaaagtagtg actaaggttg gccatggaac tggcaattta
      781 ccagtagtac aaataaattt taaggtcaat ttaccgtaag tagcatcacc ttcaccttca
      841 ccggagacag aaaatttgtg accattaaca tcaccatcta attcaaccaa aattgggaca
      901 acaccagtga ataattcttc acctttagag gatcccattt ttgagggaat attcaactgt
      961 ttttttttat catgttgatg ctctgcataa
//
