LOCUS       sequence-161817-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TEF1 promoter with 
            yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pITGFP89
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..783
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac
       61 aaaatgggat cctctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
      121 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
      181 acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg
      241 ccaaccttag tcactacttt cggttatggt gttcaatgtt ttgcgagata cccagatcat
      301 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
      361 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
      421 accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta
      481 ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa
      541 aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa
      601 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
      661 aaccattact tatccactca atctgcctta tccaaagatc caaacgaaaa gagagaccac
      721 atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac
      781 aaagcatcca acttgaacat ttcgagaaag cttaccatat caaccccatc atgctctttc
      841 gaaaattcaa atagcacatc cattccttcg cccgcttcct catctcaaag ccacactcca
      901 atgagaaaca tgagctcact ctctgataac agcgttttca gccggaatat ggaacaatca
      961 tcaccaatca ctccaagtat gtaccaattt ggtcagcagc agtcaaacag tatatgtggt
     1021 agcaccgtta gtgtgaatag t
//
