LOCUS       sequence-161822-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of membrane 
            targeted-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-myr-GFP
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             32..73
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..106,107..820)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(101..103,104..106,107..817)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(146..167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(407..426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     754..775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(820..844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             852..875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             879..902
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatatcaaca agtttgtaca aaaaagttgg catggggagc agcaagagca agcccaagga
       61 ccccagccag cgccggcgca ggcctggatc cgcggccgcc atggtgagca agggcgagga
      121 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      181 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      241 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      301 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      361 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      421 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      481 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      541 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      601 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      661 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      721 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      781 cgccgggatc actctcggca tggacgagct gtacaagtaa cccaactttc ttgtacaaag
      841 tggttgatat cgactacaag gacgacgatg acaagatcga ctacaaggac gacgatgaca
      901 agatcgacta caaggacgac gatgacaatc catcacactg gcggccgctc taaatcgata
      961 gtttgtttga atgaggcttc agtactttac ag
//
