LOCUS       sequence-161823-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yEGFP-ERG9 ER signal peptide under the control of TEF1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers 
            CE
  TITLE     A squalene synthase protein degradation method for improved 
            sesquiterpene production in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. 
            Epub 2016 Dec 8.
  PUBMED    27939849
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-01"; volume: "39:209-219. doi"; pages: " 
            10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8"
COMMENT     Sequence Label: pILGFPJ1
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(91..504)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     protein_bind    715..748
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcaccatcta attcaaccaa aattgggaca acaccagtga ataattcttc acctttagag
       61 gatcccattt tctcgagttt gtaattaaaa cttagattag attgctatgc tttctttcta
      121 atgagcaaga agtaaaaaaa gttgtaatag aacaagaaaa atgaaactga aacttgagaa
      181 attgaagacc gtttattaac ttaaatatca atgggaggtc atcgaaagag aaaaaaatca
      241 aaaaaaaaaa ttttcaagaa aaagaaacgt gataaaaatt tttattgcct ttttcgacga
      301 agaaaaagaa acgaggcggt ctcttttttc ttttccaaac ctttagtacg ggtaattaac
      361 gacaccctag aggaagaaag aggggaaatt tagtatgctg tgcttgggtg ttttgaagtg
      421 gtacggcgat gcgcggagtc cgagaaaatc tggaagagta aaaaaggagt agaaacattt
      481 tgaagctatg gtgtgtgggg gatcacttgt gggggattgg gtgtgatgta aggattcgcg
      541 gtcctcgaaa attaaaagtc caacgcgcct gttgcttcct atgtgatatg tattatatgt
      601 aatatgcata aatatatcta ctgcattgta ttttgaacgt acaaagtatg cattgtttat
      661 acgctattat cagccaaagt tgggtggtcg ctttctggtc gagcaaccct taatataact
      721 tcgtataatg tatgctatac gaagttatta ggtctagaga tcccaataca acagatcacg
      781 tgatcttttt gtaagatgaa gttgaagtag tgttgcaccg tgccaatgca ggtggctatt
      841 agattaaata tgtgatttgt tctattaagt ttcctgtata attaatgggg agcgctgatt
      901 ctcttttggt acgcttccca tccagcattt ctgtatcttt caccttcaac cttaggatct
      961 ctacccttgg cgaaaagtcc tctgccaaca atgatgatat ctgatccacc acttac
//
