LOCUS       sequence-161824-        4311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ATF2, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ATF2_WT
FEATURES             Location/Qualifiers
     source          1..4311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1711..1810)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1827..1846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1828..1846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1851..1867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1851..1867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2298..3089
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3182..3770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3671..3690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3924..3941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4100..4127)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4219..4305)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgaaattc aagttacatg tgaattctgc caggcaatac aaggacctgt
      241 ggaatatgag tgatgacaaa ccctttctat gtactgcgcc tggatgtggc cagcgtttta
      301 ccaacgagga tcatttggct gtccataaac ataaacatga gatgacactg aaatttggtc
      361 cagcacgtaa tgacagtgtc attgtggctg atcagacccc aacaccaaca agattcttga
      421 aaaactgtga agaagtgggt ttgtttaatg agttggcgag tccatttgag aatgaattca
      481 agaaagcttc agaagatgac attaaaaaaa tgcctctaga tttatcccct cttgcaacac
      541 ctatcataag aagcaaaatt gaggagcctt ctgttgtaga aacaactcac caggatagtc
      601 ctttacctca cccagagtct actaccagtg atgagaagga agtaccattg gcacaaactg
      661 cacagcccac atcagctatt gttcgtccag catcattaca ggttcccaat gtgctgctta
      721 caagttctga ctcaagtgta attattcagc aggcagtacc ttcaccaacc tcaagtactg
      781 taatcaccca ggcaccatcc tctaacaggc caattgtccc tgtaccaggc ccatttcctc
      841 ttctgttaca tcttcctaat ggacaaacca tgcctgttgc tattcctgca tcaattacaa
      901 gttctaatgt gcatgttcca gctgcagtcc cactcgttcg accagtcacc atggtgccta
      961 gtgttccagg aatcccaggt ccttcctctc cccaaccagt acagtcagaa gcaaaaatga
     1021 gattaaaagc tgctttgacc cagcaacatc ctccagttac caatggtgat actgtcaaag
     1081 gtcatggtag cggattggtt aggactcagt cagaggaatc tcgaccgcag tcattacaac
     1141 agccagccac atccactaca gaaactccgg cttctccagc tcacacaact ccacagaccc
     1201 aaagtacaag tggtcgtcgg agaagagcag ctaacgaaga tcctgatgaa aaaaggagaa
     1261 agtttttaga gcgaaataga gcagcagctt caagatgccg acaaaaaagg aaagtctggg
     1321 ttcagtcttt agagaagaaa gctgaagact tgagttcatt aaatggtcag ctgcagagtg
     1381 aagtcaccct gctgagaaat gaagtggcac agctgaaaca gcttcttctg gctcataaag
     1441 attgccctgt aaccgccatg cagaagaaat ctggctatca tactgctgat aaagatgata
     1501 gttcagaaga catttcagtg ccgagtagtc cacatacaga agctatacag catagttcgg
     1561 tcagcacatc caatggagtc agttcaacct ccaaggcaga agctgtagcc acttcagtcc
     1621 tcacccagat ggcggaccag agtacagagc ctgctctttc acagatcgtt atggctcctt
     1681 cctcccagtc acagccctca ggaagttgat tgccaacttt cttgtacaaa gttggcatta
     1741 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1801 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1861 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1921 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1981 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2041 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2101 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2161 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2221 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2281 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2341 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2401 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2461 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2521 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2581 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2641 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2701 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2761 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2821 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2881 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2941 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3001 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3061 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3121 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3841 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3901 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3961 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4021 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4081 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4141 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4201 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4261 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
