LOCUS       sequence-161826-        4218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM45 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM45_p.M126I
FEATURES             Location/Qualifiers
     source          1..4218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2097..2196)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2213..2232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2214..2232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2237..2253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2237..2253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2684..3475
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3568..4156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4057..4076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atggacgaag ctggcagctc tgcgagcggc gggggcttcc gcccgggcgt
      721 ggacagcctg gacgaaccgc ccaacagccg catcttcctt gtgatcagca agtacacacc
      781 tgagtcggtg ctgagggagc gcttctcgcc ttttggcgac atccaggaca tctgggtggt
      841 gcgggacaag cacaccaagg agtccaaggg cattgctttc gtcaagttcg cccgcagctc
      901 acaggcctgc agggccatgg aggagatgca tggccagtgc ctcggcccca acgacaccaa
      961 gcccatcaag gttttcattg ctcagtcccg atcatctgga agtcaccgag atgttgaaga
     1021 tgaagaactt acaagaatct ttgttataat accaaagtcc tacacagaag aagatctgcg
     1081 ggaaaaattt aaggtgtatg gagatatcga gtattgcagc attattaaga ataaagtgac
     1141 tggagaaagt aaaggtttgg gctacgtacg atacttaaaa ccatcacaag ctgcccaagc
     1201 aatagaaaac tgtgatcgaa gttttagagc aatcttggct gaacctaaaa ataaagcatc
     1261 tgaatcctct gaacaagatt attatagtaa tatgaggcaa gaagctttgg gacatgaacc
     1321 tagagtaaat atgtttccat ttgaacaaca atctgaattt tcaagttttg acaagaatga
     1381 tagccgaggc caggaagcaa tctccaaacg cttgtcagtt gtatcaagag ttcctttcac
     1441 tgaagaacag cttttcagca tttttgatat agtaccagga ttggaatatt gtgaagttca
     1501 acgagatcct tattcaaatt atggtcatgg agtggttcag tattttaatg tagcatcagc
     1561 tatttatgca aaatacaaat tacatggatt tcagtaccct cctgggaacc gaataggtgt
     1621 ttccttcatt gatgatggaa gtaatgcaac agatctcctt agaaaaatgg caacacagat
     1681 ggtagctgca cagcttgcat caatggtgtg gaataaccca agtcagcaac aatttatgca
     1741 atttggagga agctctggat cacagttgcc tcaaatccag acagatgttg tacttccatc
     1801 atgcaaaaaa aaagctcctg ctgaaactcc tgtgaaagaa agacttttta ttgtgtttaa
     1861 tcctcatcct ttacctttag acgtattaga agatatattc tgtcgttttg gtaacctgat
     1921 cgaagtttac cttgtgtcag gaaaaaatgt ggggtatgcc aagtatgccg atagaataag
     1981 tgctaatgat gccattgcca ctctacatgg aaagattctg aatggggtga gacttaaagt
     2041 tatgctggca gattcgccaa gagaagaatc taacaaacgg caaagaactt actgattgcc
     2101 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2161 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2221 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2281 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2341 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2401 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2461 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2521 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2581 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2641 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2701 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2761 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2821 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2881 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2941 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3001 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3061 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3121 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3181 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3241 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3301 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3361 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3421 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3481 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4201 gccttttgct cacatgtt
//
