LOCUS       sequence-161827-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of membrane 
            targeted-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-myr-GFP
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        128..146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     ncRNA           complement(241..367)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(395..409)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(437..558)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(623..646)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(650..673)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    681..705
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(750..771)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctgagcgtcc gggcggaagg atcaggacgc tcgctgcgcc cttcgtctga cgtggcagcg
       61 ctcgccgtga ggaggggggc gcccgcggga ggcgccaaaa cccggcgcgg aggccacgcg
      121 tcctaggtaa tacgactcac tatagggccc gcccgcaaac aacatcaaac aaaccagcag
      181 acatatgcaa caagacacga gacggagtaa tggccggccg cattagtctt ccaattgaaa
      241 aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca aagagatagc
      301 aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt gaattccgag
      361 gcagtaggca ctctcgcttg ggcgagagta agtacatctg tggcttcact acttactctc
      421 gcccaagcga gatcgctcac tgtcaacagc aatatacctt ctcgagcctt ctgttgggtt
      481 aacctgaaga agtaatccca gcaagtgttt ccaagatgtg caggcaacga ttctgtaaag
      541 tactgaagcc tcattcaaac aaactatcga tttagagcgg ccgccagtgt gatggattgt
      601 catcgtcgtc cttgtagtcg atcttgtcat cgtcgtcctt gtagtcgatc ttgtcatcgt
      661 cgtccttgta gtcgatatca accactttgt acaagaaagt tgggttactt gtacagctcg
      721 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      781 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
      841 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      901 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      961 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     1021 ccgtcctcct tgaa
//
