LOCUS       sequence-161839-        4092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STK11, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_STK11_p.G251V
FEATURES             Location/Qualifiers
     source          1..4092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1971..2070)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2087..2106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2088..2106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2111..2127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2111..2127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2558..3349
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gaggtggtgg acccgcagca gctgggcatg ttcacggagg gcgagctgat
      721 gtcggtgggt atggacacgt tcatccaccg catcgactcc accgaggtca tctaccagcc
      781 gcgccgcaag cgggccaagc tcatcggcaa gtacctgatg ggggacctgc tgggggaagg
      841 ctcttacggc aaggtgaagg aggtgctgga ctcggagacg ctgtgcagga gggccgtcaa
      901 gatcctcaag aagaagaagt tgcgaaggat ccccaacggg gaggccaacg tgaagaagga
      961 aattcaacta ctgaggaggt tacggcacaa aaatgtcatc cagctggtgg atgtgttata
     1021 caacgaagag aagcagaaaa tgtatatggt gatggagtac tgcgtgtgtg gcatgcagga
     1081 aatgctggac agcgtgccgg agaagcgttt cccagtgtgc caggcccacg ggtacttctg
     1141 tcagctgatt gacggcctgg agtacctgca tagccagggc attgtgcaca aggacatcaa
     1201 gccggggaac ctgctgctca ccaccggtgg caccctcaaa atctccgacc tgggcgtggc
     1261 cgaggcactg cacccgttcg cggcggacga cacctgccgg accagccagg gctccccggc
     1321 tttccagccg cccgagattg ccaacggcct ggacaccttc tccggcttca aggtggacat
     1381 ctggtcggct ggggtcaccc tctacaacat caccacggtt ctgtacccct tcgaagggga
     1441 caacatctac aagttgtttg agaacatcgg gaaggggagc tacgccatcc cgggcgactg
     1501 tggccccccg ctctctgacc tgctgaaagg gatgcttgag tacgaaccgg ccaagaggtt
     1561 ctccatccgg cagatccggc agcacagctg gttccggaag aaacatcctc cggctgaagc
     1621 accagtgccc atcccaccga gcccagacac caaggaccgg tggcgcagca tgactgtggt
     1681 gccgtacttg gaggacctgc acggcgcgga cgaggacgag gacctcttcg acatcgagga
     1741 tgacatcatc tacactcagg acttcacggt gcccggacag gtcccagaag aggaggccag
     1801 tcacaatgga cagcgccggg gcctccccaa ggccgtgtgt atgaacggca cagaggcggc
     1861 gcagctgagc accaaatcca gggcggaggg ccgggccccc aaccctgccc gcaaggcctg
     1921 ctccgccagc agcaagatcc gccggctgtc ggcctgcaag cagcagtgat tgccaacttt
     1981 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2041 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2101 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2161 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2221 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2281 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2341 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2401 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2461 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2521 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     2581 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2641 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     2701 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2761 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2821 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2881 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2941 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3001 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3061 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3121 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3181 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3241 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3301 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     3361 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg tt
//
