LOCUS       sequence-161843-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNAs, compatible with double-shRNA 
            expression, EF1alpha promoter, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Kampmann M, Bassik MC, Weissman JS
  TITLE     Integrated platform for genome-wide screening and construction of 
            high-density genetic interaction maps in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):E2317-26. doi: 
            10.1073/pnas.1307002110. Epub 2013 Jun 5.
  PUBMED    23739767
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307002110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-06-18- 18"; volume: "110"; issue: "25"; pages: 
            "E2317-26"
COMMENT     Sequence Label: pMK1200
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(141..155)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(239..943)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(517..536)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     786..804
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacatgtctc gacctggctt actagtcggt tcaactctag atatgtggta tagatcggaa
       61 gagcgtcgtg tagggaaaga gtgtgactga ccccttgaag cccgaggccc aaggcagtgg
      121 gcaggtcctg aagttcatta catctgtggc ttcactaatg aacttcagac ctgcccaacg
      181 ctctgggtca acagcaatat accttcttct cgagtcaagg cgaataccta ggcaattctt
      241 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt
      301 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
      361 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
      421 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
      481 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
      541 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
      601 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
      661 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
      721 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
      781 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
      841 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
      901 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctca
//
