LOCUS       sequence-161845-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNAs, compatible with double-shRNA 
            expression, SFFV promoter, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, 
            Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS
  TITLE     Next-generation libraries for robust RNA interference-based 
            genome-wide screens.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3384-91. doi: 
            10.1073/pnas.1508821112. Epub 2015 Jun 15.
  PUBMED    26080438
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1508821112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-06-30- 30"; volume: "112"; issue: "26"; pages: 
            "E3384-91"
COMMENT     Sequence Label: pMK1221
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(148..162)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(532..551)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acccttaaac atgtctcgac ctggcttact agtcggttca actctagata gaagaacaag
       61 atcggaagag cgtcgtgtag ggaaagagtg tgactgaccc cttgaagtcc gaggcccaag
      121 gcagtgggca ggtcctgaag ttcattacat ctgtggcttc actaatgaac ttcagacctg
      181 cccaacgctc tgggtcaaca gcaatatacc ttcttctcga gtccaataaa gttcctaggc
      241 aattcctgca ggcttacttg tacagctcgt ccattcctcc ggtggagtgc cgtccctcgg
      301 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
      361 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
      421 cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tctcctttca
      481 gagcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
      541 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
      601 cgccgtcctg gagagaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
      661 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg
      721 cggggtgc
//
