LOCUS       sequence-161847-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNAs, compatible with double-shRNA 
            expression, SFFV promoter, BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, 
            Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS
  TITLE     Next-generation libraries for robust RNA interference-based 
            genome-wide screens.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3384-91. doi: 
            10.1073/pnas.1508821112. Epub 2015 Jun 15.
  PUBMED    26080438
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1508821112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-06-30- 30"; volume: "112"; issue: "26"; pages: 
            "E3384-91"
COMMENT     Sequence Label: pMK1224
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(141..155)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aacatgtctc gacctggctt actagtcggt tcaactctag agcgaatgta gagatcggaa
       61 gagcgtcgtg tagggaaaga gtgtgactga ccccttgaag tccgaggccc aaggcagtgg
      121 gcaggtcctg aagttcatta catctgtggc ttcactaatg aacttcagac ctgcccaacg
      181 ctctgggtca acagcaatat accttcttct cgagtcagat actcatccta ggcaattcct
      241 gcaggcttaa ttaagtttgt gccccagttt gctagggagg tcgcagtatc tggccactgc
      301 cacctcgtgc tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt ccagtctgta
      361 gtccacatag tagacgccag gcatcttgag gttcttagcg ggtttcttgg atctatatgt
      421 ggtcttgatg tttgcgatca gatggctccc gcccacgagc ttcagggcca tgtcgtttct
      481 gccttccagg ccgccgtcag cggggtacag cgtctcggtg aaggcctccc agccgagtgt
      541 tttcttctgc atcacagggc cgttggatgt gaagttcacc cctctgatct tgacgttgta
      601 gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc
      661 gtatgtggtg actctctccc atgtgaagcc ctcagggaag gactgcttga agaagtcggg
      721 gatgccctgt gtgtggttga tgaaggtctt gctgccgtag aggaagctag tagccaggat
      781 gtcgaaagcg aaggggagag ggccgccctc gaccaccttg attctcatgg tctgggtgcc
      841 ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tgatggttgt ccacggtgcc
      901 ctccatgtac agcttcatgt gcatgttctc cttaatcagc
//
