LOCUS       sequence-161865-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lacI in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Unnikrishnan A, Gafken PR, Tsukiyama T
  TITLE     Dynamic changes in histone acetylation regulate origins of DNA 
            replication.
  JOURNAL   Nat Struct Mol Biol. 2010 Apr;17(4):430-7. doi: 10.1038/nsmb.1780. 
            Epub 2010 Mar 14.
  PUBMED    20228802
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1780";
            journalName: "Nat Struct Mol Biol"; date: "2010-04"; volume: "17"; 
            issue: "4"; pages: "430-7"
COMMENT     Sequence Label: pRS406-CMV-lacI-3FLAG
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             220..291
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     promoter        372..390
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     372..389
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    441..474
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(521..541)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(521..539)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(560..576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(573..595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
       61 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      121 gggcagggat cctgtactcc accaaagaag aagagaaagg ttgccgagct cggtggatct
      181 ggcggtagac agctcggtgg atctggcggt agacagctcg attataaaga tgacgatgac
      241 aaggattata aagatgacga tgacaaggat tataaagatg acgatgacaa gtaactgcag
      301 gaatttatgg acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
      361 atacacatac gatttaggtg acactataga acgcggccgc cagctgaagc ttcgtacgct
      421 gcaggtcgac aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
      481 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
      541 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      601 ttccacacaa catacgagcc ggaagcataa agtgta
//
