LOCUS       sequence-161866-        4665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KEAP1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_KEAP1_p.G524C
FEATURES             Location/Qualifiers
     source          1..4665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2544..2643)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2660..2679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2661..2679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2684..2700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2684..2700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3131..3922
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4015..4603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4504..4523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct attctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaag
      661 agttggcatg cagccagatc ccaggcctag cggggctggg gcctgctgcc gattcctgcc
      721 cctgcagtca cagtgccctg agggggcagg ggacgcggtg atgtacgcct ccactgagtg
      781 caaggcggag gtgacgccct cccagcatgg caaccgcacc ttcagctaca ccctggagga
      841 tcataccaag caggcctttg gcatcatgaa cgagctgcgg ctcagccagc agctgtgtga
      901 cgtcacactg caggtcaagt accaggatgc accggccgcc cagttcatgg cccacaaggt
      961 ggtgctggcc tcatccagcc ctgtcttcaa ggccatgttc accaacgggc tgcgggagca
     1021 gggcatggag gtggtgtcca ttgagggtat ccaccccaag gtcatggagc gcctcattga
     1081 attcgcctac acggcctcca tctccatggg cgagaagtgt gtcctccacg tcatgaacgg
     1141 tgctgtcatg taccagatcg acagcgttgt ccgtgcctgc agtgacttcc tggtgcagca
     1201 gctggacccc agcaatgcca tcggcatcgc caacttcgct gagcagattg gctgtgtgga
     1261 gttgcaccag cgtgcccggg agtacatcta catgcatttt ggggaggtgg ccaagcaaga
     1321 ggagttcttc aacctgtccc actgccaact ggtgaccctc atcagccggg acgacctgaa
     1381 cgtgcgctgc gagtccgagg tcttccacgc ctgcatcaac tgggtcaagt acgactgcga
     1441 acagcgacgg ttctacgtcc aggcgctgct gcgggccgtg cgctgccact cgttgacgcc
     1501 gaacttcctg cagatgcagc tgcagaagtg cgagatcctg cagtccgact cccgctgcaa
     1561 ggactacctg gtcaagatct tcgaggagct caccctgcac aagcccacgc aggtgatgcc
     1621 ctgccgggcg cccaaggtgg gccgcctgat ctacaccgcg ggcggctact tccgacagtc
     1681 gctcagctac ctggaggctt acaaccccag tgacggcacc tggctccggt tggcggacct
     1741 gcaggtgccg cggagcggcc tggccggctg cgtggtgggc gggctgttgt acgccgtggg
     1801 cggcaggaac aactcgcccg acggcaacac cgactccagc gccctggact gttacaaccc
     1861 catgaccaat cagtggtcgc cctgcgcccc catgagcgtg ccccgtaacc gcatcggggt
     1921 gggggtcatc gatggccaca tctatgccgt cggcggctcc cacggctgca tccaccacaa
     1981 cagtgtggag aggtatgagc cagagcggga tgagtggcac ttggtggccc caatgctgac
     2041 acgaaggatc ggggtgggcg tggctgtcct caatcgtctc ctttatgccg tggggggctt
     2101 tgacgggaca aaccgcctta attcagctga gtgttactac ccagagagga acgagtggcg
     2161 aatgatcaca gcaatgaaca ccatccgaag cggggcaggc gtctgcgtcc tgcacaactg
     2221 tatctatgct gctgggtgct atgatggtca ggaccagctg aacagcgtgg agcgctacga
     2281 tgtggaaaca gagacgtgga ctttcgtagc ccccatgaag caccggcgaa gtgccctggg
     2341 gatcactgtc caccagggga gaatctacgt ccttggaggc tatgatggtc acacgttcct
     2401 ggacagtgtg gagtgttacg acccagatac agacacctgg agcgaggtga cccgaatgac
     2461 atcgggccgg agtggggtgg gcgtggctgt caccatggag ccctgccgga agcagattga
     2521 ccagcagaac tgtaccagtt gattgccaac tttcttgtac aaagttgtca ttataagaaa
     2581 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2641 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2701 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2761 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     2821 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     2881 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     2941 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3001 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3061 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3121 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3181 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3241 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3301 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3361 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3421 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3481 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3541 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3601 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3661 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     3721 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     3781 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     3841 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     3901 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     3961 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4141 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
