LOCUS       sequence-161869-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the Clover gene to the GAPDH locus of human cells. 
            Clover is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Clover-Muro
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     379..400
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(446..499)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
       61 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      121 tcttgtaggt accgtcgtcc ttgaaagaga tggtgcgctc ctggacgtag ccttcgggca
      181 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcaggcca
      241 cgccgtagcc gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      301 agatgaactt cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg ccgcggacgc
      361 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      421 acagctcctc gcccttgctc accatcgggc caggattctc ctccacgtca ccgcatgtta
      481 gaagacttcc tctgccctct cctccggacc cgccgccacg cgccattccc tccttggagg
      541 ccatgtgggc catgaggtcc accaccctgt tgctgtagcc aaattcgttg tcatacctag
      601 aagatgaaaa gagttgtcag ggcccttttt ctgagccagc caccagaggg cgccagaccc
      661 tgcacttttt aagagccagt ctctggcccc agccacatac caggaaatga gcttgacaaa
      721 gtggtcgttg agggcaatgc cagccccagc gtcaaaggtg gaggagtggg tgtcgctgtt
      781 gaagtcagag gagaccacct ggtgctcagt gtagcccagg atgcccttga
//
