LOCUS       sequence-161875-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway donor vector for ARF19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-ARF19
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3939..4038)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4055..4074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4056..4074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4079..4095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4079..4095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4197..4216)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4208..5017
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4275..4294)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5164..5752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5653..5672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc aaaatgaaag ctccatcaaa tggatttctt ccaagttcca acgaaggaga
      721 gaagaagcca atcaattctc aactatggca cgcttgtgca gggcctttag tttcattacc
      781 tcctgtggga agtcttgtgg tttacttccc tcaaggacac agcgagcaag ttgcagcatc
      841 gatgcagaag caaacagatt ttataccaaa ttacccaaat cttccttcta agctgatttg
      901 cttgcttcac agtgttacat tacatgctga taccgaaaca gatgaagtct atgcacaaat
      961 gactcttcaa cctgtgaata agtatgatag agaagcattg ctagcttctg atatgggctt
     1021 gaagctaaac agacaaccta ctgagttttt ttgcaagact cttactgcaa gtgacacaag
     1081 cactcatggt ggattctctg taccgcgtcg tgcagctgag aaaatattcc ctcctcttga
     1141 tttctcgatg caaccgcctg cgcaagagat tgtagctaaa gatttacatg atactacatg
     1201 gactttcaga catatctatc gaggccaacc aaaaagacac ttgcttacca caggttggag
     1261 cgtttttgtt agcacaaaga gactatttgc gggtgattca gttttgtttg taagagatga
     1321 gaaatcacag ctgatgttgg gtataagacg tgcaaataga caaactccga ctctttcctc
     1381 atcggtcata tccagcgaca gtatgcacat tgggatactt gcagctgcag ctcatgctaa
     1441 tgccaatagt agccctttta ccatcttctt caatccaagg gcaagtcctt cagagtttgt
     1501 agttccttta gccaaataca acaaagcctt atacgctcaa gtatctctag gaatgagatt
     1561 ccggatgatg tttgagactg aggattgtgg ggttcgtaga tatatgggta cagtcacagg
     1621 tattagtgat cttgaccctg taagatggaa aggctcacaa tggcgtaatc ttcaggtagg
     1681 atgggatgaa tcaacagctg gagataggcc aagccgagta tccatatggg aaatcgaacc
     1741 cgtcataact cctttttaca tatgtcctcc tccatttttc agacctaagt acccgaggca
     1801 acccgggatg ccagatgatg agttagacat ggaaaatgct ttcaaaagag caatgccttg
     1861 gatgggagaa gactttggga tgaaggacgc acagagttcg atgttccctg gtttaagtct
     1921 agttcaatgg atgagtatgc agcaaaacaa tccattgtca ggttctgcta ctcctcagct
     1981 cccgtccgcg ctctcatctt ttaacctacc aaacaatttt gcttccaacg acccttccaa
     2041 gctgttgaac ttccaatccc caaacctctc ttccgcaaat tcccaattca acaaaccgaa
     2101 cacggttaac catatcagcc aacagatgca agcacaacca gccatggtga aatctcaaca
     2161 acaacaacaa caacaacaac aacaacacca acaccaacaa caacaactgc aacaacaaca
     2221 acaactacag atgtcacagc aacaggtgca gcaacaaggg atttataaca atggtacgat
     2281 tgctgttgct aaccaagtct cttgtcaaag tccaaaccaa cctactggat tctctcagtc
     2341 tcagcttcag cagcagtcaa tgctccctac tggtgctaaa atgacacacc agaacataaa
     2401 ttctatgggg aataaaggct tgtctcaaat gacatcgttt gcgcaagaaa tgcagtttca
     2461 gcagcaactg gaaatgcata acagtagcca gttattaaga aaccagcaag aacagtcctc
     2521 tctccattca ttacaacaaa atctgtccca aaatcctcag caactccaaa tgcaacaaca
     2581 atcatcaaaa ccaagtcctt cacaacagct tcagttgcag ctactgcaga agctacagca
     2641 gcagcaacag cagcagtcga ttcctccagt aagctcatcc ttacagccac aattatcagc
     2701 gttgcagcag acacaaagcc atcaattgca acaacttctg tcgtctcaaa atcaacagcc
     2761 cttggcacat ggtaataaca gcttcccagc ttcaactttc atgcagcctc cacagattca
     2821 ggtgagtcct cagcagcaag gacagatgag taacaaaaat cttgtagccg ctggaagatc
     2881 acattctggc cacacagatg gagaagctcc ttcttgttca acctcacctt ccgccaataa
     2941 cacgggacat gataatgttt caccgacaaa tttcctgagc agaaatcaac agcaaggaca
     3001 agctgcatct gtatctgcat ctgattcagt ctttgagcgc gcaagcaatc cggtccaaga
     3061 gctttataca aaaactgaga gccggatcag tcaaggcatg atgaatatga agagtgctgg
     3121 tgaacatttc agatttaaaa gcgcggtaac agatcaaatc gatgtatcca cagcgggaac
     3181 gacgtactgt cctgatgttg ttggccctgt acagcagcaa caaactttcc cactaccatc
     3241 atttggtttt gatggagact gccaatctca tcatccaaga aacaacttag ctttccctgg
     3301 taatctcgaa gccgtaactt ctgatccact ctattctcaa aaggactttc aaaacttggt
     3361 tcccaactat ggcaacacac caagagacat tgagacggag ctgtccagtg ctgcaatcag
     3421 ttctcagtca tttggtattc ccagcattcc ctttaagccc ggatgttcaa atgaggttgg
     3481 cggcatcaat gattcaggaa tcatgaatgg tggaggactg tggcccaatc agactcaacg
     3541 aatgcgaaca tatacaaagg ttcaaaaacg agggtcagta ggtagatcaa tagatgttac
     3601 ccgttatagc ggctatgatg aacttaggca tgacttagcg agaatgtttg gcatcgaagg
     3661 acagctcgaa gatccgctaa cctctgattg gaaactcgtc tacaccgatc acgaaaacga
     3721 tattttacta gttggtgatg atccttggga agagtttgtg aactgcgtgc agaacataaa
     3781 gatactatca tcagtagaag ttcagcaaat gagcttagac ggagatcttg cagctatccc
     3841 aaccacaaac caagcctgca gcgaaacaga cagcggaaat gcttggaaag tacactatga
     3901 agacacttct gctgcagctt ctttcaacag atagtaacac ccagctttct tgtacaaagt
     3961 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     4021 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     4081 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     4141 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     4201 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     4261 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     4321 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     4381 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     4441 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     4501 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     4561 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     4621 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     4681 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     4741 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     4801 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     4861 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     4921 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     4981 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     5041 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     5101 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5281 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5401 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5461 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5521 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5581 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5641 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5701 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5761 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
