LOCUS       sequence-161885-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PPP2R1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PPP2R1A_p.W140C
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2439..2538)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2555..2574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2556..2574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2579..2595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2579..2595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3026..3817
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3910..4498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4399..4418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcggcggccg acggcgacga ctcgctgtac cccatcgcgg tgctcataga
      721 cgaactccgc aatgaggacg ttcagcttcg cctcaacagc atcaagaagc tgtccaccat
      781 cgccttggcc cttggggttg aaaggacccg aagtgagctt ctgcctttcc ttacagatac
      841 catctatgat gaagatgagg tcctcctggc cctggcagaa cagctgggaa ccttcactac
      901 cctggtggga ggcccagagt acgtgcactg cctgctgcca ccgctggagt cgctggccac
      961 agtggaggag acagtggtgc gggacaaggc agtggagtcc ttacgggcca tctcacacga
     1021 gcactcgccc tctgacctgg aggcgcactt tgtgccgcta gtgaagcggc tggcgggcgg
     1081 cgactgtttc acctcccgca cctcggcctg cggcctcttc tccgtctgct acccccgagt
     1141 gtccagtgct gtgaaggcgg aacttcgaca gtacttccgg aacctgtgct cagatgacac
     1201 ccccatggtg cggcgggccg cagcctccaa gctgggggag tttgccaagg tgctggagct
     1261 ggacaacgtc aagagtgaga tcatccccat gttctccaac ctggcctctg acgagcagga
     1321 ctcggtgcgg ctgctggcgg tggaggcgtg cgtgaacatc gcccagcttc tgccccagga
     1381 ggatctggag gccctggtga tgcccactct gcgccaggcc gctgaagaca agtcctggcg
     1441 cgtccgctac atggtggctg acaagttcac agagctccag aaagcagtgg ggcctgagat
     1501 caccaagaca gacctggtcc ctgccttcca gaacctgatg aaagactgtg aggccgaggt
     1561 gagggccgca gcctcccaca aggtcaaaga gttctgtgaa aacctctcag ctgactgtcg
     1621 ggagaatgtg atcatgtccc agatcttgcc ctgcatcaag gagctggtgt ccgatgccaa
     1681 ccaacatgtc aagtctgccc tggcctcagt catcatgggt ctctctccca tcttgggcaa
     1741 agacaacacc atcgagcacc tcttgcccct cttcctggct cagctgaagg atgagtgccc
     1801 tgaggtacgg ctgaacatca tctctaacct ggactgtgtg aacgaggtga ttggcatccg
     1861 gcagctgtcc cagtccctgc tccctgccat tgtggagctg gctgaggacg ccaagtggcg
     1921 ggtgcggctg gccatcattg agtacatgcc cctcctggct ggacagctgg gagtggagtt
     1981 ctttgatgag aaacttaact ccttgtgcat ggcctggctt gtggatcatg tatatgccat
     2041 ccgcgaggca gccaccagca acctgaagaa gctagtggaa aagtttggga aggagtgggc
     2101 ccatgccaca atcatcccca aggtcttggc catgtccgga gaccccaact acctgcaccg
     2161 catgactacg ctcttctgca tcaatgtgct gtctgaggtc tgtgggcagg acatcaccac
     2221 caagcacatg ctacccacgg ttctgcgcat ggctggggac ccggttgcca atgtccgctt
     2281 caatgtggcc aagtctctgc agaagatagg gcccatcctg gacaacagca ccttgcagag
     2341 tgaagtcaag cccatcctag agaagctgac ccaggaccag gatgtggacg tcaaatactt
     2401 tgcccaggag gctctgactg ttctgtctct cgcctgattg ccaactttct tgtacaaagt
     2461 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2521 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2581 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2641 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2701 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2761 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2821 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2881 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2941 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     3001 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3061 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3121 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3181 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3241 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3301 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3361 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3421 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3481 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3541 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3601 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3661 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3721 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3781 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     3841 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4021 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
