LOCUS       sequence-161889-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA19 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA19_no stop
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(692..791)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(808..827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(809..827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttcaaaa tggagaagga aggactcggg
      121 cttgagataa cggagctgag attggggctt ccggggagag atgtggcaga gaagatgatg
      181 aagaagagag ctttcacgga gatgaatatg acgtcgtcgg gtagtaatag tgatcaatgt
      241 gaaagcggcg tcgtttcatc tggtggtgac gctgagaagg ttaatgattc gccggcggcg
      301 aaaagccagg tggtggggtg gccaccggtt tgttcttacc ggaagaaaaa cagctgtaag
      361 gaagcttcga ccacgaaagt ggggttaggg tatgtgaaag tgagcatgga tggtgtgcct
      421 tatttgagga agatggatct tggttcgagc caaggctatg atgatctagc ctttgctctt
      481 gataagctct tcggtttccg tggcatcggt gtggccttga aagatggtga caactgcgaa
      541 tacgttacca tatacgaaga caaagatgga gactggatgc tcgccggtga tgtaccttgg
      601 gggatgtttc tagagtcatg caagaggttg agaataatga aaagatcgga tgctaccggg
      661 tttgggctgc agcctagagg agtagacgag cacccagctt tcttgtacaa agttggcatt
      721 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      781 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
      841 gttt
//
