LOCUS       sequence-161890-        4584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF8, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DCAF8_p.E63K
FEATURES             Location/Qualifiers
     source          1..4584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2463..2562)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2579..2598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2580..2598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2603..2619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2603..2619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3050..3841
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3934..4522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4423..4442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atgtccagca aagggagcag cacagatggc agaacagact tagctaatgg
      721 aagcctgtct agcagtccag aggagatgtc tggagctgaa gaggggaggg agacatcctc
      781 aggcattgaa gtggaggcct cagacctgag tttgagcttg actggggatg atggtggccc
      841 caaccgcacc agcacaaaaa gtcgaggcac agacacagag agctcaggtg aagataagga
      901 ctctgacagc atggaggaca ctggtcatta ctccattaat gatgaaaatc gagtccatga
      961 ccgctcagag gaagaggaag aggaggaaga agaggaggaa gaagagcagc ctcggcgccg
     1021 tgtacagcgc aagcgggcta accgtgacca ggactcatca gatgatgagc gggccctaga
     1081 ggactgggtg tcctcagaaa catcagctct accccgacct cgctggcaag ccctccctgc
     1141 ccttcgggag cgggagctgg gttcaagtgc ccgctttgtc tatgaggcct gtggggcaag
     1201 agtctttgtg cagcgtttcc gcctgcagca tgggcttgag ggccatactg gttgtgtcaa
     1261 taccctgcac tttaaccagc gcggcacctg gctggccagt ggcagcgatg acctgaaggt
     1321 ggtggtgtgg gattgggtac ggcggcagcc agtactggac tttgagagtg gccacaaaag
     1381 taatgtgttc caggccaagt ttcttcctaa cagtggtgat tctactctgg ccatgtgtgc
     1441 ccgtgacggg caggttcgag tagcagaact gtctgccaca cagtgttgca agaatacaaa
     1501 acgtgtggcc cagcacaagg gagcgtccca caagttggca ctggaaccag actctccctg
     1561 tacgttctta tctgcaggtg aagatgcagt tgttttcacc attgacctga gacaagaccg
     1621 cccagcgtcg aaactggtgg tgacaaaaga gaaagagaag aaagtggggc tgtatacgat
     1681 ctatgtgaat cctgccaata cccaccagtt tgcagtgggt ggacgagatc agtttgtaag
     1741 gatttatgac cagaggaaaa ttgatgagaa tgagaacaat ggagtactca agaagttctg
     1801 tcctcatcac ctggtgaaca gtgagtccaa agcaaacatc acctgtcttg tgtacagcca
     1861 cgacggcaca gagctcctgg ccagttacaa tgatgaagac atttacctct tcaactcctc
     1921 tcacagtgat ggggcccagt atgttaagag atacaagggc cacagaaata atgccacagt
     1981 aaaaggcgtc aatttctatg gccccaagag tgagtttgtg gtgagcggta gtgactgtgg
     2041 gcacatcttc ctctgggaga aatcatcctg ccagattatt cagttcatgg agggggacaa
     2101 gggaggcgtg gtaaactgtc ttgagcccca ccctcacctg cctgtgctgg caaccagtgg
     2161 cctagaccat gatgtgaaga tctgggcacc cacagctgaa gcttccactg agctgacagg
     2221 gttaaaagat gtgattaaga agaacaagcg ggagcgggat gaagatagct tgcaccaaac
     2281 tgacctgttt gatagtcaca tgctgtggtt ccttatgcat cacctgagac agagacgcca
     2341 tcaccggcgc tggcgagaac ctggggttgg ggccacagac gcggactctg atgagtctcc
     2401 cagctcctca gacacatcgg acgaggagga gggccctgac cgggtgcagt gcatgccatc
     2461 tttgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2521 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2581 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2641 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2701 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     2761 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2821 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2881 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2941 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     3001 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     3061 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     3121 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     3181 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     3241 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     3301 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     3361 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     3421 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     3481 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3541 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3601 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3661 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3721 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3781 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3841 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4081 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4561 ttgctggcct tttgctcaca tgtt
//
