LOCUS       sequence-161894-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA20 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA20_no stop
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(626..725)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(742..761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(766..782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(766..782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttcaaaa tgggaagagg gagaagttca
      121 tcgtcttctt caatagagag tagcagcaag agcaacccat tcggtgcttc gtcaagtact
      181 cgaaacctaa gcacggacct gagactcgga ctcagcttcg ggacatcctc agggactcaa
      241 tatttcaacg gtggctatgg gtactccgtt gcagctccgg cggtagagga tgcggaatat
      301 gtggcggctg tggaggagga agaagagaat gagtgtaaca gtgtagggag cttttacgtg
      361 aaagtgaaca tggaaggagt tccaattgga agaaagatcg atctaatgtc tcttaatggc
      421 taccgcgact tgatcagaac ccttgatttc atgttcaacg catccattct ctgggctgaa
      481 gaagaagaca tgtgcaatga gaagagtcac gtgctaacgt acgcagataa ggaaggtgac
      541 tggatgatgg ttggagatgt tccttgggag atgttcttgt ctactgtgag aagactgaag
      601 atttcaagag ctaattacca ctaccaccca gctttcttgt acaaagttgg cattataaga
      661 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      721 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
      781 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaata
//
