LOCUS       sequence-161902-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA3 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA3_no stop
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(667..766)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(783..802)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(784..802)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(807..823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gcttcaaaat ggatgagttt gttaacctca
      121 aggaaacaga gctgaggctg ggattaccgg gaacagataa tgtatgtgaa gcaaaagaga
      181 gagtttcttg ctgtaataac aacaataaga gagtactatc aactgatact gagaaggaga
      241 ttgaatcatc atcaaggaaa actgaaacat cccctcctcg aaaggctcag attgttggat
      301 ggccaccagt tagatcttac aggaagaaca acattcagag taagaagaat gaatctgagc
      361 atgagggtca aggaatctat gtgaaagtaa gtatggatgg tgcaccatac ttgaggaaaa
      421 tagatctgag ttgttacaaa ggatactcag agttgcttaa agctttagaa gtgatgttca
      481 aattctctgt gggagagtac tttgagagag atggatataa aggttcagac tttgtgccta
      541 cttatgaaga caaagatggt gattggatgc tcattggtga tgttccatgg gagatgttca
      601 tatgtacgtg caagagacta aggatcatga aaggatcaga agccaaaggt ttaggctgtg
      661 gtgtacaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
      721 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
      781 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
      841 ctcaaaatct ctgatgttac
//
