LOCUS       sequence-161907-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector for IAA5 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA5_no stop
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(590..689)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(706..725)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(707..725)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(730..746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttcaaaa tggcgaatga gagtaataat
      121 cttggactcg aaatcaccga actacggcta ggtcttcccg gagatatcgt cgtctccggt
      181 gagtccatct ccgggaagaa gagggcttct ccggaagtag agattgattt gaaatgtgaa
      241 ccggcgaaaa agagtcaagt tgtgggttgg ccaccggttt gttcgtaccg gagaaagaac
      301 agtctcgaac ggaccaaaag ttcgtacgtg aaagtgagtg tagatggagc tgcattttta
      361 aggaagattg atttggaaat gtacaaatgt taccaagatc ttgcttccgc tctgcaaatt
      421 ctgttcggat gctatatcaa ttttgatgat acgttgaagg aaagtgaatg tgtaccaata
      481 tatgaagaca aagatggaga ttggatgctt gctggagatg ttccttggga aatgttcctt
      541 ggatcgtgca agaggttaag gattatgaag agatcatgta acagaggaca cccagctttc
      601 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
      661 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
      721 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      781 tacattgcac aagataaa
//
