LOCUS       sequence-161913-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF8, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DCAF8_p.G588C
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2466..2565)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2582..2601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2583..2601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2606..2622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2606..2622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3053..3844
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3937..4525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4426..4445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtccagca aagggagcag cacagatggc agaacagact tagctaatgg
      721 aagcctgtct agcagtccag aggagatgtc tggagctgaa gaggggaggg agacatcctc
      781 aggcattgaa gtggaggcct cagacctgag tttgagcttg actggggatg atggtggccc
      841 caaccgcacc agcacagaaa gtcgaggcac agacacagag agctcaggtg aagataagga
      901 ctctgacagc atggaggaca ctggtcatta ctccattaat gatgaaaatc gagtccatga
      961 ccgctcagag gaagaggaag aggaggaaga agaggaggaa gaagagcagc ctcggcgccg
     1021 tgtacagcgc aagcgggcta accgtgacca ggactcatca gatgatgagc gggccctaga
     1081 ggactgggtg tcctcagaaa catcagctct accccgacct cgctggcaag ccctccctgc
     1141 ccttcgggag cgggagctgg gttcaagtgc ccgctttgtc tatgaggcct gtggggcaag
     1201 agtctttgtg cagcgtttcc gcctgcagca tgggcttgag ggccatactg gttgtgtcaa
     1261 taccctgcac tttaaccagc gcggcacctg gctggccagt ggcagcgatg acctgaaggt
     1321 ggtggtgtgg gattgggtac ggcggcagcc agtactggac tttgagagtg gccacaaaag
     1381 taatgtgttc caggccaagt ttcttcctaa cagtggtgat tctactctgg ccatgtgtgc
     1441 ccgtgacggg caggttcgag tagcagaact gtctgccaca cagtgttgca agaatacaaa
     1501 acgtgtggcc cagcacaagg gagcgtccca caagttggca ctggaaccag actctccctg
     1561 tacgttctta tctgcaggtg aagatgcagt tgttttcacc attgacctga gacaagaccg
     1621 cccagcgtcg aaactggtgg tgacaaaaga gaaagagaag aaagtggggc tgtatacgat
     1681 ctatgtgaat cctgccaata cccaccagtt tgcagtgggt ggacgagatc agtttgtaag
     1741 gatttatgac cagaggaaaa ttgatgagaa tgagaacaat ggagtactca agaagttctg
     1801 tcctcatcac ctggtgaaca gtgagtccaa agcaaacatc acctgtcttg tgtacagcca
     1861 cgacggcaca gagctcctgg ccagttacaa tgatgaagac atttacctct tcaactcctc
     1921 tcacagtgat ggggcccagt atgttaagag atacaagggc cacagaaata atgccacagt
     1981 aaaaggcgtc aatttctatg gccccaagag tgagtttgtg gtgagcggta gtgactgtgg
     2041 gcacatcttc ctctgggaga aatcatcctg ccagattatt cagttcatgg agggggacaa
     2101 gggaggcgtg gtaaactgtc ttgagcccca ccctcacctg cctgtgctgg caaccagtgg
     2161 cctagaccat gatgtgaaga tctgggcacc cacagctgaa gcttccactg agctgacagg
     2221 gttaaaagat gtgattaaga agaacaagcg ggagcgggat gaagatagct tgcaccaaac
     2281 tgacctgttt gatagtcaca tgctgtggtt ccttatgcat cacctgagac agagacgcca
     2341 tcaccggcgc tggcgagaac ctggggttgg ggccacagac gcggactctg atgagtctcc
     2401 cagctcctca gacacatcgg acgaggagga gtgccctgac cgggtgcagt gcatgccatc
     2461 ttgattgcca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2521 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2581 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2641 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     2701 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     2761 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     2821 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2881 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     2941 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     3001 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     3061 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3121 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3181 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3241 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3301 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3361 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3421 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3481 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3541 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3601 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     3661 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     3721 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     3781 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     3841 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4561 cttttgctgg ccttttgctc acatgtt
//
