LOCUS       sequence-161922-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of LacZ with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 LacZ-V5 blast
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(55..77)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 actgtcggga tcaacaagtt tgtacaaaaa agttggcacc atgatagatc ccgtcgtttt
       61 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      121 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      181 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
      241 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
      301 gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
      361 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
      421 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
      481 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
      541 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
      601 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
      661 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg c
//
